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    Antidiabetic and Hypolipidaemic Effects of Citrus aurantifolin Leaves on Hyperglycaemic and diabetic Rats
    (UOFK, 2015-02) Howeida, Mustafa A. ; Idris, Eltayeb B. ; Almahdi, Ali M. ; . Sania, Shaddad A ; Abdelwahhab, Mohammad H.
    Objectives: This research aimed to study the hypoglycaemic and hypolipidaemic effects of aqueous and methanolic extracts of Citrus aurantifolin leaves, in hyperglycaemic (type II) and Streptozotocin diabetic rats (type I). Methodology: This plant was phytochemically screened using the standard methods for determination of its chemical composition., The hypolipidaemic and hypoglycaemic effects were determined following the Glucose tolerance test (GTT) model and the results were compared to the control and the reference drugs Insulin and Glibenclamide for types I and II diabetes mellitus respectively. Results: Phytochemical screening of C.aurantifolin revealed presence of sterols (++), alkaloids (+), fla64rfc 64rfc 4wsazThe results of this research in type II showed that both extracts of C.aurantifolin exhibited an early persistent hypoglycaemic effect in type II hyperglycaemic rats as compared to the control. Both doses of the aqueous extract and dose 400 mg ∕ kg of the methanolic extract reduced blood glucose significantly (P <0.001) throughout the experiment, while dose 200 mg ∕ kg of the methanolic extract reduced blood glucose significantly (P<0.05) throughout the experiment. In type I diabetic rats, the hypoglycaemic effect was slow but highly significant (P <0.001) as it only started at the 4th hour post dosing. Regarding the effect of C.aurantifolin on blood cholesterol, the aqueous extract showed an earlier, less persistent and highly significant (P <0.001) hypocholesterolaemic effect in type II hyperglycaemic rats than the methanolic extract as its effect occured at the 1st hour only while dose 400 mg/kg of the methanolic reduced cholesterol level significantly (P<0.05), at the 2nd and 4th hours and dose 200 mg/kg at the 1st and 2nd hours post dosing. In type I diabetic rats, dose 400 mg/kg and Insulin,showed a significant (P <0.05) hypocholesterlaemic effect at the 4th hour post dosing, the effect of the extract continued to the 8th hour. The highest significant reduction (P <0.001) was exhibited by dose 200mg/kg of the methanolic extract at the 4th hour post dosing. Concerning blood triglycerides, both the aqueous and methanolic extracts of C.aurantifolin, reduced blood triglycerides significantly (P <0.001) and (P<0.05),at the 2nd and 4th hours post dosing, respectivly.In Type I diabetic rats,the effect of the aqueous extract was highly significant (P <0.001) since the 1st hour and continued throughout the experiment. The methanolic extract, reduced blood triglycerides significantly (P<0.05) 8 and 12 hours post dosing while the effect of Insulin was significant (P<0.05) since the 1st hour and continued throughout the experiment. In conclusion the leaves of C.aurantifolin confirmed its traditional use in herbal medicine as a hypoglycaemic and hypolipidaemic agent which can be very effective.