University of Khartoum

Effect of some Addenda Manipulation on Micropropagation of Musa sp.cv. Grand Naine

Effect of some Addenda Manipulation on Micropropagation of Musa sp.cv. Grand Naine

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Title: Effect of some Addenda Manipulation on Micropropagation of Musa sp.cv. Grand Naine
Author: Ali Morfeine Aika, Ekhlas
Abstract: This research was carried out at the Tissue culture Laboratory of Sudan University of Science and Technology for manipulation, problems of oxidation at initiation phase, for the study of the role of some chemicals and nonchemical addenda to the nutrient media, to explore the optimum concentration of growth regulators of multiplication in vitro, rooting. The research also included the study of the effects of decapitation sectioning, light intensity, physical status on proliferation of banana. Number of shoots per explant, length of shoot, number of roots per explants , length of root, were recorded. Results of manipulation problems of oxidation at initiation phase indicated that the pretreatment of dipping in, solutions of citric+ascorbic acid, ginger and activated charcoal gave positive results to counteract oxidation. Also addenda to the media of activated charcoal, and citric + ascorbic acid were satisfactory. The intact explant showed better results than vertical sectioning. Phytagel as a gelling agent was resistant to oxidation compared with Agar Agar and Agar Agar + Phytagel. Low light intensity was better than high in the reduction of browning. The role of some chemicals and nonchemical addenda to the nutrient media resulted that the sucrose at 30 g/l and 45 g/l and glucose at 45g/l and 60g/l show significant increase in multiplication. Amino acids supplements at 200g/l indicates significant increase in multiplication but suppressed rooting. vi Organic acid supplements were not enhancive except oxalic acid. Sodium meta-bisulfate at 10mg/l enhanced significant increase in multiplication, but at 20 mg favoured rooting. Silver nitrate at 0.1 mg/l enhanced significant increase in multiplication. Vitamin (Biotein) supplements were not enhancive. Results to explore the optimum concentration of growth regulators show that the thidiazuron at 0.5 mg/l, 1 mg/l and 2mg/l gave significant increase in multiplication but reduced length of leaf and rooting. Indole acetic acid at 0.4 mg/l with Benzylamino purinc enhanced significant increase in multiplication but at 0.8 mg/l favoured rooting. Naphthalene acetic acid with Benzylamino purine supplements were not enhancive but favoured rooting. Addition of indole butyric acid lead to significant increase in rooting and the best rooting was obtained at 2mg/l. Studying the effects of decapitation sectioning, physical status, light intensity on proliferation of banana, resulted that the sectioning without decapitation produced maximum significant multiplication. Different physical status did not affect the multiplication rate, but liquid and double phase medium encouraged other parameters. Cultures incubated in shelves with 100 lux (without lamp) induced significant increase in multiplication.
URI: http://khartoumspace.uofk.edu/handle/123456789/11958
Date: 2015-06-14


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