University of Khartoum

Evaluation of fumigant action of garlic (allium sativum) aqueous extract against the cowpea seed weevil callosobruchus maculatus (f.) Under laboratory conditions

Evaluation of fumigant action of garlic (allium sativum) aqueous extract against the cowpea seed weevil callosobruchus maculatus (f.) Under laboratory conditions

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Title: Evaluation of fumigant action of garlic (allium sativum) aqueous extract against the cowpea seed weevil callosobruchus maculatus (f.) Under laboratory conditions
Author: Abd Elaziz Sulieman Ahmed Ishag, Ishag
Abstract: The efficacy of the fumigant action of garlic aqueous ethyl acetate extract (95:5 v/v) from the Chinese cultivar was tested against the adult stage of the cowpea weevil, Callosobruchus maculatus (F.) (Coleoptera: Bruchidae), under laboratory conditions at the Department of Crop Protection, Faculty of Agriculture, University of Khartoum, Shambat, Sudan. Garlic extracts were prepared by 24 hrs soaking of garlic (Chinese cultivar) powder in water: ethyl acetate solution (95:5% v/v). The bioassay was done in both plastic cups (200 ml) and jute sacs (100g) placed in glass chambers with sliding doors. The tested concentrations were 0.1%, 1%, 5% and 10%. The results indicated that the water: ethyl acetate extract was as equally effective as steam distilled or readymade extracts. The median lethal doses (LD50s) for the extracts, after 6, 12, 24, 48 and 72 hours, were 0.351%, 1.14%, 0.26%, 0.088% and 0.059% in plastic cups and 0.59%, 1.38%, 0.44%, 0.38% and 0.12% in jute sacs. The corresponding LD90 values were 14.92%, 7.41%, 1.66%, 2.99% and 1.20% in cups and 35%, 17.79%, 7.12%, 6.50% and 1.92 % in sacs. The corresponding values of median lethal times (LT50) at the concentrations of 10%, 5%, 1% and 0.1% were 3.56, 4.35, 9.87, 50.63 for plastic cups and 12.27, 13.9, 34, 68 hours for jute sacs. The respective LT90s were 16.32, 19.09, 63.86, 112.39 and 6.926, 22.54, 53.05, 52.68 hours. Mortality increased with increase in exposure period and concentration. The responses of test insects to fumigation were homogenous as indicated by the steep slope of the probit lines. Based on the current results, 90% mortality can be achieved at the concentration of 5% within 24 hrs exposure in jute sacs and the corresponding values for plastic cups were even lower. No significant recoveries were observed for 4 days following exposure and few non-significant cases of malformation were observed
Description: 62 page
URI: http://khartoumspace.uofk.edu/handle/123456789/12773
Date: 2015-06-15


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