University of Khartoum

Evaluation of Genetic Diversity and Drought Tolerance in Wheat (Triticum aestivum L.) Cultivars using Molecular Markers

Evaluation of Genetic Diversity and Drought Tolerance in Wheat (Triticum aestivum L.) Cultivars using Molecular Markers

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dc.contributor.advisor Dr. Adil Ali El-Hussein. en_US
dc.contributor.author Hind Emad Fadoul, Abd Elgadir
dc.date.accessioned 2015-06-16T09:02:31Z
dc.date.available 2015-06-16T09:02:31Z
dc.date.issued 2015-06-16
dc.date.submitted 2012
dc.identifier.uri http://khartoumspace.uofk.edu/handle/123456789/13028
dc.description 93 page
dc.description.abstract Abstract The main objective of this study was to assess the genetic diversity among locally cultivated wheat cultivars and identification of molecular markers associated with drought tolerance for use in breeding programs aiming at developing drought tolerant cultivars. Diversity was estimated by Random Amplified Polymorphic DNA (RAPD) technique using 21 arbitrary 10-mer and six Dehydration Responsive Elements (DRE) primers. Seed storage protein diversity was also assessed by Sodium Dodecyl Sulfate-Poly Acrylamide Gel Electrophoresis (SDS-PAGE). Drought tolerance was assessed under greenhouse conditions at different water regimes and Stress Susceptibility Index (SSI) was calculated for each cultivar. RAPD analysis detected a total of 232 DNA fragments of which 152 (65.5%) were polymorphic. Detected genetic similarity matrices (based on Dice index) ranged from 0.435 to 0.81. These indices were used to construct a dendrogram using average linkage between groups. Wheat cultivars were clustered into six groups; Cluster A included cultivars Debaira, Nisr and Sasraib, cluster B included Nabta, Wadi Elniel, Argeen and Condor, cluster C included the cultivar Imam, cluster D contained the cultivar Al-neelain, cluster E included Tagana and Bohain and the most distant cultivar (Khalifa) in the dendrogram stands in cluster F. The highest similarity (0.81) among the wheat cultivars was observed between Debaira and Nisr while the lowest (0.043) was between Alneelain and Bohain. Unique DNA fragments of different sizes were detected in a particular cultivar but not in the others. Such fragments could be used as markers for their respective cultivars. For example: one positive plus six negative markers were detected for cultivar Tagana, four negative markers for Nisr, two negative markers for Khalifa and one marker was detected for each of cultivars Condor, Argeen, Imam, Al-neelain and Bohain. However, no marker was observed for the cultivars Nabta, Wadi Elneil and Sasraib. The SDS-PAGE patterns of seed storage proteins from the tested wheat cultivars showed eight different protein subunits, five (62.5%) of which were polymorphic. One of the protein subunits was unique to cultivar Argeen and so can be used as a marker for this cultivar. The highest similarity index (1.00) was observed between 4 pairs of wheat cultivars. The lowest (0.60) was observed between 2 pairs of cultivars. As high similarity indices (0.60 to 1.00) were recorded for protein analysis, the overall degree of variation is relatively low. The highest value of calculated stress susceptibility index (SSI) was recorded for cultivar Tagana (SSI=1.86) while the lowest was recorded for Nabta (-1.4). RAPD-PCR and SDS-PAGE techniques showed considerable potential for estimating genetic diversity among our wheat cultivars. The information reported here will have important implications in the development of drought tolerant wheat cultivars with wider and diverse genetic background to achieve improved wheat productivity. en_US
dc.publisher UOFK en_US
dc.subject Evaluation of Genetic Diversity and Drought Tolerance in Wheat (Triticum aestivum L.) Cultivars using Molecular Markers en_US
dc.title Evaluation of Genetic Diversity and Drought Tolerance in Wheat (Triticum aestivum L.) Cultivars using Molecular Markers en_US
dc.type Thesis en_US
dc.Degree M.Sc en_US
dc.Faculty Science en_US
dc.contributor.faculty Botany en_US

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