University of Khartoum

In vitro Response of Pineapple (Ananas comosus L. Merr.) to Medium Formulae, Stature and Benzyladenine Cytokinin

In vitro Response of Pineapple (Ananas comosus L. Merr.) to Medium Formulae, Stature and Benzyladenine Cytokinin

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Title: In vitro Response of Pineapple (Ananas comosus L. Merr.) to Medium Formulae, Stature and Benzyladenine Cytokinin
Author: Elhag Adam, Dalia
Abstract: This study was conducted to investigate medium formulation, medium stature and benzyladenine concentrations on the growth and rooting of pineapple plantlets grown in vitro. Two media formulations, that is Murashige and Skoog (1962) (MS) and Gamborg (1968) (Gamborg B5) in solid and liquid forms, and five concentrations of BA (0, 2, 3, 4 and 5 mg/l) were used, and 15 explants were used per treatment. Number and length of shoots and roots, and number of nodes were recorded. The results indicated that the MS medium in liquid state with 3 mg/l and 4 mg/l BA resulted in significantly higher number of shoot and nodes, respectively, compared with MS in solid state and Gamborg B5 medium in both forms. The solidified MS medium, free of growth regulators, on the other hand, resulted in significantly higher number of roots than liquid MS medium and Gamborg B5 medium in both forms. Gamborg B5 medium in liquid state without BA resulted in significantly higher shoot and root length than solid state and Murashige and Skoog medium in both forms. High BA concentrations depressed number and length of shoot and root but enhanced node number. The high BA concentrations (4 and 5 mg/l) tended to promote callus formation at a later stage of the experiment (9-12 weeks of culture). Generally, the performance of explants in liquid media was better than in solid media.
Description: 113page
URI: http://khartoumspace.uofk.edu/handle/123456789/13261
Date: 2015-06-16


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