University of Khartoum

Chemotaxonomic Profiling of Flavonoids of TwoAbutilon spp (Hambuk)

Chemotaxonomic Profiling of Flavonoids of TwoAbutilon spp (Hambuk)

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Title: Chemotaxonomic Profiling of Flavonoids of TwoAbutilon spp (Hambuk)
Author: Elamin, Mai Hassan
Abstract: This study was conducted for screenining of flavonoids in the genus Abutilon (Hambuk) in two species indigenous to Sudan (Abutilon pannosum and Abutilon figarianum) by subjecting them to chemotaxonomic study and biological screening implementing different chromatographic analysis methods (TLC, HPLC and HPLC-MS/MS). In addition to antioxidant activity screening for the two species of the genus Abutilon (Hambuk). The chromatographic analysis (TLC) revealed the accumulation of flavonoids, flavonoidal acids and other phenolic acids in the ethyl acetate fractions of the studied parts of the two Abutilon species. The HPLC-DAD proved the presence of flavonoids and flavonoidal acids in the two Abutilon spp studied. The flavonoids detected were mainly flavones, flavnols and anthocyanins. HPLC-MS/MS CID experiment of ethyl acetate fractions of A. figarianum leaves recorded the presence of quercetin glycoside, kaempferol glucuronide, kaempferol diglycosides rhamnosyl, kaempferol glycoside, quercetin glucuronide, luteolin glucuronide and luteolin glycoside. While the A. pannosum showed luteolin glycoside, apigenin, apigenin glycoside, apigenin glucuronide and flavone glucuronide. Additionally A. pannosum flowers contains, delphinidin diglycosides, cyanidin diglycosides, myricetin diglycosides, myricetin glycoside, quercetin diglycosides, quercetin glycoside, kaempferol glucuronide, kaempferol diglycosides, quercetin glycoside malonide, kaempferol glycoside, myricetin glucuronide, quercetin glucuronide, apigenin glucuronide, kaempferol glycoside malonide, naringenin glucuronide and luteolin glycosides. A. figarianum flowers possess quercetin glycosides, kaempferol glucuronide, quercetin glycoside malonide, kaempferol glycoside, myricetin glucuronide, quercetin glucuronide, kaempferol glucoside malonide, luteolin glycosides and phenolic acids derivatives. Regarding flavonoids as chemotaxonomic markers A. pannosum flowers contained the largest number of flavonoids (21 compounds); A. pannosum and A. figarianum flowers shared the following flavonoids, quercetin glycoside, kaempferol glucuronide, quercetin glycoside malonide, kaempferol glycoside, myricetin glucuronide, quercetin glucuronide, kaempferol glycoside malonide and luteolin glycoside. Anthocyanins (delphinidin diglycosides and cyanidin diglycosides), myricitin diglycosides, myricitin glycoside, quercetin diglycosides, kaempferol diglycosides, apigenin glucuronide, naringenin glucuronide and quercetin glucuronide were found only in A. pannosum flowers. The following compounds were shared between leaves of A. pannosum and A. figarianum, kaempferol diglycosides rhamnosyl, kaempferol glycoside and luteolin glycoside. Apigenin and Apigenin derivatives were found only in A. pannosum leaves. While the kaempferol glucuronide, luteolin glucuronide and quercetin derivatives were found only in A. figarianum leaves. Kaempferol glycoside, quercetin glucuronide and luteolin glycoside were detected in all studied parts of both A. pannosum and A. figarianum. In vitro antioxidant activity screening revealed that the ethyl acetate extract of the tested parts of the two Abutilon spp showed significant inhibition of DPPH radicals at a concentration of 12.5 _6;g /ml (flowers 80%, leaves 65%) the petroleum ether extracts showed slight activity and chloroform extract had no activity at the same concentration.
Description: 92 Pages
URI: http://khartoumspace.uofk.edu/handle/123456789/13397
Date: 2015-06-17


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