University of Khartoum

Tribolium Castaneum And Tribolium Confusum

Tribolium Castaneum And Tribolium Confusum

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Title: Tribolium Castaneum And Tribolium Confusum
Author: Bakr, Ali Gabir Albushari
Abstract: The flour beetles Tribolium species are cosmopolitan. Although a major pest of stored grains, the beetles received little attention in Sudan. For the purpose of this study samples of Tribolium were collected from different geographical regions. The study revealed the presence of two species in Sudan: Tribolium castaneum and Tribolium confusum. Tribolium confusum was found to be less abundant (28.5%). The populations of Tribolium castaneum were found to harbor different mutants. Of the 1400 adult samples of Tribolium castaneum, 87 (6.2%) showed one or more mutations. The types of mutants detected were pygmy (py), cephalothorax (Cx), body colour (bc), antennal segments (sa), blunt abdominal and metathoracic projection (Bamp), space between eyes (sbe) and the size of head mutant (soh). The highest rate of mutation was reported from Juba (25%) compared to 5% and less in all other regions while there was absence of mutants from Kosti samples. Cytogenetic findings from this study confirm the conserved karyotype of Tribolium castaneum where the karyotype illustrated presence of ten (10) pairs of chromosomes. Nine (9) pairs were autosomes and one pair of sex chromosomes. The confused flour beetle Tribolium confusum has only 9 pairs of chromosomes. Amylase enzyme profiles, for populations of the seven geographical regions, were investigated using polyacylamide gel electrophoresis, enzyme polymorphism was detected. Differences in gene expression of the expressed protein were reported. There was dominance of amy1 phenotype in all populations. expression of the amylase allele (amy1) and its co-expression with amy2 in the heterozygote (amy1,2) morphs was detected in both T. castaneum and T. confusum. The differential expression between T. castaneum and T. confusum was observed; however, there was a similarity between the expression of the amylase in adults and larvae in each of the two species. The amylase gene was expressed differentially in beetles from different geographical regions The study used the available literature to draw conclusions and make recommendations for the future research of Tribolium in Sudan
URI: http://khartoumspace.uofk.edu/handle/123456789/13583
Date: 2015-06-17


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