University of Khartoum

Taxonomy, Fatty Acids Pattern and Protein Profiles of Staphylococcus species Isolated from Abscesses in Sheep

Taxonomy, Fatty Acids Pattern and Protein Profiles of Staphylococcus species Isolated from Abscesses in Sheep

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Title: Taxonomy, Fatty Acids Pattern and Protein Profiles of Staphylococcus species Isolated from Abscesses in Sheep
Author: Noura Karamalla Mohamed Salih, Salih
Abstract: The causative agents of Morel's disease were identified according to their biochemical properties into twelve different species of Staphylococci, viz: Staph. aureus subsp. anaerobius, (26%), Staph. hyicl (22%), Staph. caseolylicus (20%), Staph. aureus (5%), Staph. succhurolyticus (5%), Staph. dephini (5%), Staph. sciuri (4%), Staph. hominis (4%), Staph. carnosus (3%), Staph. intermedius (3%), Staph. cohni (3%) and Staph. xylosus (2%). The best medium that supports luxuriant growth was found to be Cooked Meat Media (5.0x 10x) CFU/ml) and the next one was Brai in Hea Infusion (Dirco) (3.4x 10x CFU /ml) with respect to growth rate, all the phases of growth in all the species were found to be similar, but Staph. carnosus had a long lag phase, weak and long logarithmic phase in comparison with the other species. The effects of phenol and formalin on these organisms were studied. Phenol had a very good inhibition effect on these species at concentration of I% which killed the bacteria in less than 10 minutes and after nine hours at low concentration of 0.05%. Formalin acted only weakly at a concentration of 1%, while there was still growth after eleven hours. The fatty acids patterns of all the species available were analyzed using Gas Liquid Chromatography. The patterns differed from one species to the other, and some fatty acids were presented in one species but not the others, e.g. Staph. clllreus subsp. werobills was the only species which had Cv) and While CII was present only in Staph. reseolyticus. The findings are helpful in identification of these species. According to their protein profiles, the twelve species (Staphylococci isolated from sheep abscesses were grouped into four groups. Members of group one which contains the coagulase-positive species viz: Staph. clureus subsp. clncierobius, Staph. clllreus, Staph. ltyicus, Staph lule/puini and Staph. inlerl/ledius, they had a large number protein bands ranging between 21-31 bands. The species in the other three groups (coagulase-negative and either mannose-positive or negative) has less than eight bands. Therefore, it appears that there were large number of protein band in the coagulase positive Staphylococci.
URI: http://khartoumspace.uofk.edu/handle/123456789/13841
Date: 2015-06-22


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