University of Khartoum

Immunization of Calves against Experimental Bovine Schistosomiasis Using Glutathione-S-Transferase and Keyhole Limpet Haemocyanin

Immunization of Calves against Experimental Bovine Schistosomiasis Using Glutathione-S-Transferase and Keyhole Limpet Haemocyanin

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dc.contributor.advisor Hamid Omer Bushara en_US
dc.contributor.author El Sayed Ishag El Shafea, El Shafea
dc.date.accessioned 2015-06-22T09:39:41Z
dc.date.available 2015-06-22T09:39:41Z
dc.date.issued 2015-06-22
dc.date.submitted 1997
dc.identifier.uri http://khartoumspace.uofk.edu/handle/123456789/13866
dc.description.abstract The enzymes recombinant Glutathione -s- transferase (Rec. G,S,T), Native GST and the glycoprotein Keyhole limpet Haemocyanin (KLH) were subcutaneously and intramuscularly injected in different sites in the animal body to study their safety, efficacy and potency to produce immunity against experimental infection with Schistosoma bovis. Forty Zebu calves were divided into four equal groups A, B, C, and D. Group A calves were injected with three doses of Recombinant GST. Group B calves were injected with three doses of Native GST, Group C calves were injected with a combination of Rec. GST plus KLH (Two doses). Group D calves were left as controls. The vaccinated groups together with the controls were challenged by S. bovis cercariae at a dose rate of 10.000 per calf. Faecal egg count was reduced compared to the controls by 23% in group A, 16% and 22% in Group B and C, respectively. Hatchability of eggs was also lowered to about 49%, 53% and 44% in group A, B, and C, respectively than that obtained from the controls. Eggs recovered from different tissues was reduced to 56% in group A, 53% in group B and 58% in group C compared to the controls. It was observed that calcified (black) eggs constituted 62%, 48% and 50% of the eggs counted in the livers, small and large intestines of the vaccinated animals. The total worm recovery in all animals' groups, including the controls, was almost the same. There was, also, slight difference in the packed cell volume values and no difference in the haemoglobin concentrations. Using ELISA all vaccinated animals showed highly significant differences in titre till the second week after challenge, then declined and started again to rise in all groups, including controls, starting from week five after challenge onwards till the end of the experiment. en_US
dc.language.iso en en_US
dc.publisher UOFK en_US
dc.subject Immunization of Calves against Experimental Bovine Schistosomiasis Using Glutathione-S-Transferase and Keyhole Limpet Haemocyanin en_US
dc.title Immunization of Calves against Experimental Bovine Schistosomiasis Using Glutathione-S-Transferase and Keyhole Limpet Haemocyanin en_US
dc.type Thesis en_US
dc.Degree M.Sc en_US
dc.Faculty Veterinary Science en_US
dc.contributor.faculty Microbiology en_US

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