University of Khartoum

Clinical, Haematological and Serum Chemical Studies in Cross Bred Calves Naturally Infected with Theileria annulata

Clinical, Haematological and Serum Chemical Studies in Cross Bred Calves Naturally Infected with Theileria annulata

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Title: Clinical, Haematological and Serum Chemical Studies in Cross Bred Calves Naturally Infected with Theileria annulata
Author: Mohamed Toum El Haj Ramadan Adam, Adam
Abstract: The aim of this study was to evaluate serum chemistry and haematological picture of 80 cross-bred calves naturally infected with Theileria annulata compared with healthy control calves and treated calves. T. annulata was identified using lymph node biopsy smears, blood smears and identification of the vector Hyalomma anatolicum anatolicum. The lymph node biopsy smears and blood smears were fixed with absolute methanol and stained with 10% Giemsa's stain. Eight infected calves from both sex and of age that ranged between 1 and 2 months were investigated. Fourteen healthy calves were used as healthy control calves. Twenty of the eighty calves were treated with buparvaquone, oxytetracycline and Novasul to determine effect of these drugs against the disease. Calves, which did not reveal Theileria spp. schizonts in lymph nodes, were not included. All T. annulata-infected calves showed pyrexia averaged of 41.40oC, anorexia, pale mucous membranes, enlargement of superficial lymph nodes, nasal and ocular discharges, laboured respiration (average of 80 cycles/min), and tachycardia (average of 107 beats/min). 75 calves (93.75%) showed diarrhoea, 25 calves (31.25%) expressed icteric mucous membranes and bilateral exophthalmia. T. annulata infected calves showed high schizont parasitosis in their lymph nodes and piroplasms in their erythrocytes with parasitaemia average of 13%. Fatality rate among the infected calves was 21.25% and the incidence of the disease was 40%. H. a. anatolicum was collected from all the infected calves The healthy control calves did not reveal any of the former observations. T. annulata-infected calves showed low values of the haematological parameters compared with the control calves. The infected calves revealed average packed cell volume (PCV) values of 15.88%, average erythrocyte (RBC) count of 4.65 106 cell/µL, average total leukocytes (WBC) count of 2.88, 103 cell/µL, average haemoglobin (Hb) concentration of 5.84 g/dl, average mean corpuscular volume (MCV) of 33.85fl, average mean corpuscular haemoglobin (MCH) of 12.6 pg, average mean corpuscular haemoglobin concentration (MCHC) of 37.54g/dl. The control calves showed PCV values average of 28.07%, Hb values average of 11.01g/dl, RBC values average of 8.37 106 cells/µL, MCV values average of 33.54, MCH values average of 39.32 pg., WBC counts average of 5.31x103 cell/µL. The treated calves expressed PCV averaged of 22.75%, Hb average of 8.64g/dl, RBC average of 6.57x106 cell/µl, MCV average of 34.75fl, MCH average of 13.15pg, MCHC average of 37.88 g/dl, WBC average of 5.33x 103 cells/μL. T. annulata-infected calves expressed hypoprotienemia hypoaluminemia, hypocalcemia, bilirubinemia. No significant change was observed in the serum sodium and potassium values. The serum chemistry mean values of the infected calves was 6.57g/dl total protein, 2.84 g/dl albumin, 7.38 gm/dl calcium, 1.72 mg/dl bilirubin, 128.02 mEqu/L sodium, 4.4 mEqu/L potassium, 44.88 IU aspartate-aminotransferase. The control calves showed 8.07g/dl totals protein, 4.9 g/dl albumin, 11.15 mg/dl calcium, 105.35 IU/L aspartate-aminotransferase, 0.35 mg/dl total bilirubin, 130.2 mEqu/L sodium, and 3.6 mqu/L potassium. The serum chemistry mean values of the treated calves was 7.28 g/dl total protein, 3.95 g/dl albumin, 8.9 mg/dl calcium, 62 IU aspartate-aminotransferase, 0.78 mg/dl bilirubin, 127.17 mEqu/L sodium, 4.67 mEqu/L potassium.
URI: http://khartoumspace.uofk.edu/handle/123456789/14271
Date: 2015-06-23


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