University of Khartoum

Microbiological and Antibiotic Profile of Milk in Khartoum State

Microbiological and Antibiotic Profile of Milk in Khartoum State

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Title: Microbiological and Antibiotic Profile of Milk in Khartoum State
Author: Huwayda Amin Hussain, Mahmood
Abstract: This study was conducted to evaluate the quality of milk supplied to consumers from various sources during summer and winter seasons in Khartoum State. Raw milk samples were collected during winter (150 samples) and summer (100 samples) seasons. Samples were collected from Khartoum (83 samples), Khartoum North (83 samples) and Omdurman areas (84 samples). From each area samples were collected from three sources: farms, groceries and vendors. All samples were subjected to antibiotic residues test by Delvotest and to bacteriological examination (total bacterial count and Coliform count. The results showed that the highest total bacterial count was found in summer season (log10 6.895±0.678 cfu/ml), while the lowest was in winter (log10 5.563±0.572 cfu/ml). Coliform count was higher in winter (log10 3.445:±0.960 cfu/ml) and lower in summer (log10 2.684±0.418 cfu/ml). During winter season 17.3% of total samples tested were positive for antibiotic residues, while only 5% of samples were positive during summer season. The highest total bacterial count was found in Khartoum area (log10 6.245±0.921 cfu/ml), followed by Omdurman (log10 6.140±0.872 cfu/ml) and Khartoum North (log10 5.911±0.878 cfu/ml). Coliform count was high in Khartoum North (log10 3.243±0.689 cfu/ml) followed by Khartoum (log10 3.071±0.749 cfu\ml) and Omdurrnan (log10 3.051±1.01 cfu/ml). Some of samples tested for antibiotic residues in Omdurrnan area were positive (17.9%), followed by Khartoum (13.0%) and Khartoum North (6.8%). Vendors' milk had the highest total bacterial count (log10 6.182±0.963 cfu/ml) followed by farms' milk (log10 6.078±0.849 cfu/ml and groceries' milk (log10 6.024±0.890 cfu/ml). Coliform count was high in vendors' milk (Iog10 3.332±1.060 cfu/ml) followed by groceries' milk (log10 3.212±0.777 cfu/ml) and farms milk (Iog10 2.884±0.705 cfu/ml). The antibiotic residues test showed that 18.6% of venders' milk samples were positive, while 14.1% of groceries' samples were positive and 8.1% of farms' samples were positive. An experiment was conducted to determine the effect of addition of antibiotic to milk from cows under mastitis treatment as well as cows not under treatment, on the microbial quality of milk. A total of 15 cows were used in the experiment, and cows in the first group (5 cows) were under mastitis treatment, while those of the 2nd group (10 cows) were not under treatment. Milk samples (2 parts) were drawn from the first group, and the first part was directly subjected to analyses (total bacterial count, Coliform count and antibiotic residues test) while to the second part, 5 IU of penicillin were deliberately added followed by analyses. Milk from the 2nd group (first part) was directly subjected to analysis while to the 2nd part, 5 IU of penicillin was added and subjected to analysis. Results showed that total bacterial count and Coliform count were high in the first group, as well as positive samples for antibiotic residues test. Total bacterial and Coliform were counts reduced drastically in samples from the first part of the first group. Milk samples from cows not under treatment had high total bacterial and Coliform counts, while antibiotic residues test was negative. When antibiotic was added to milk from cows not under treatment, total bacterial and Coliform counts were reduced markedly in samples showing positive antibiotic residues test
URI: http://khartoumspace.uofk.edu/handle/123456789/14332
Date: 2015-06-23


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