University of Khartoum

Studies on Lipid Carbohydrate Metabolism in Camel and Sheep

Studies on Lipid Carbohydrate Metabolism in Camel and Sheep

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Title: Studies on Lipid Carbohydrate Metabolism in Camel and Sheep
Author: Nabiela Musa El Bagir, El Bagir
Abstract: Twenty-six animals were used, 14 camels and 12 sheep to investigate some aspects in lipids and carbohydrate metabolism in camel and sheep. The activities of certain lipogenic enzymes (NADP-isocitrate dehydrogenase NADP-ICDH; NADP-malate dehydrogenase NADP-MDH, glucose-6-phosphate and 6-phosphogluconate dehydrogenases G-6-PDH and 6-PGDH, ATP- citrate lyase, ATP-CCE and fatty acid synthetase FAS) were studied in the liver and three fat depόts (subcutaneous, perirenal and mesenteric) of camel and sheep. Glucose 6-phosphatase activity was estimated in the liver tissue from camel and sheep. The incorporation of acetate and glucose into fatty acids was studied in isolated adipocytes from hump and tail of the camel and sheep. Plasma lipoprotein fractions were isolated from camel plasma and their composition was demonstrated under different nutritional states. This investigation showed that the activities of all the lipogenic enzymes studied in sheep tail were higher than in the liver. The activity of NADP-ICDH was very high in all tissues studied of both camel and sheep. Camel liver showed higher values of NADP-MDH and NADP-ICDH than the adipose tissue, implies more involvement in lipid synthesis than sheep liver. The activities of NADP-MDH and ATP-CCE were very low compared to the other enzymes in both camel and sheep tissues. The camel showed similar pattern to other ruminants, having high NADP-ICDH and low ATP-CCE and NADP-MDH. Pentose cycle enzymes showed considerable activity in both camel and sheep tissues. The combined activity of G-6-PDH and 6-PGDH showed similar proportions in both camel and sheep tissues. The adipose tissue in the camel showed high capacity of fatty acid synthesis, similar to other ruminants. Incorporation of the (U-C14) acetate into fatty acids in the hump and tail adipose tissue was very high compared to D-(U-C14) glucose. Camel is similar to other ruminants in utilizing acetate rather than glucose for lipid synthesis. Glucose-6-phosphatase activity showed very low value in camel liver compared to sheep liver. Gluconeogenesis in the camel body might not be mainly a liver function. The lipoprotein fractions in the camel plasma showed similar pattern to other ruminants in having high proportion of the high density lipoprotein (HDL, d > 1.063). Fasting and quarter feeding resulted in very high concentration of the very low density lipoprotein fraction (VLDL, d < 1.006).
URI: http://khartoumspace.uofk.edu/handle/123456789/14675
Date: 2015-06-23


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