University of Khartoum

Yeast Flora in the Digestive Tract of the One-Humped Camel

Yeast Flora in the Digestive Tract of the One-Humped Camel

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Title: Yeast Flora in the Digestive Tract of the One-Humped Camel
Author: Abdel Samid, Gusi Hussein Abdel Samid
Abstract: A total of 280 samples of the one-humped camel stomach contents besides the small and large intestine contents were studied. They were collected from Tamboul (about 150 kilometer south east of Khartoum). Samples were cultivated on Sabouraud dextrose agar (pH 4) supplemented with chloramphenicol. Out of the 280 samples, 62 samples yielded yeasts (22.1 %) (one isolate per sample). According to the methods described by J. Lodder, isolates were identified and classified by performing microscopic and biochemical tests. Isolates belonged to two genera (Candida and Torulopsis).Yeasts distribution was 21 isolates from the rumen (14 C. tropicalis, 6. C. krusei, 1 C. stellatoidea), 8 isolates from the reticulum (3 C. tropicalis, 3 C. krusei, 1 C. stellatoidea 1 T. inconspicua), 12 isolates from the omasum. (5 C. tropicalis, 3 C. krusei, 1 C. luistaneiae, 2 T. inconspicua, 1 T. candida), 9 isolates from the abomasum (6 C. tropicalis, 3. C. krusei), 6 isolates from the small intestine (4 C. tropicalis, 2 C. krusei) and 6 isolates from the large intestine (3 C. tropicalis, 1 C. krusei, T. inconspicua, T. candida) The pH value of different parts of the gastrointestinal tract (G.I. T.) was studied and found to be (5-7) in the rumen, (4-6) in the reticulum, (4-5) in the omasum, (1-2) in the abomasum, (5-6) in the small intestine and (5-7) in the large intestine. A relative decrease in the number of the isolates was noticed when going down the stomach compartments associated with a decrease in the pH value. The regression coefficient between the number of isolates and the pH value was insignificant. A pathogenicity trial was done by injecting two rabbits intravenously with C. tropicalis and other two rabbits with C. krusei. The results revealed the two organisms re-isolated from the kidneys of the four rabbits. The results also revealed that sera from the four rabbits showed high levels of antibodies specific to C. tropicalis and C. krusei. A total of 250 serum samples of the camel from (Tamboul, University of Khartoum farm and Albutana) were negative when tested against whole cell, culture filltrate and somatic extract antigens using agglutination, immunodiffusion and counterimmunoelectrophoresis tests.
URI: http://khartoumspace.uofk.edu/handle/123456789/15048
Date: 2015-06-25


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