University of Khartoum

Acacia Senegal (L.) Willd: Capacity For Vegetative Propagation And Seed Physiology

Acacia Senegal (L.) Willd: Capacity For Vegetative Propagation And Seed Physiology

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Title: Acacia Senegal (L.) Willd: Capacity For Vegetative Propagation And Seed Physiology
Author: El Basheir, Yahia Hamid Ali
Abstract: Seed polymorphism (weight and size differences) in Acacia senegal (L.) willd. seed samples, collected from low and high yield trees/denoted by B and A respectively, its effects on germination percentages and shoot growth performance have been demonstrated. Seedlings grown from high yield seed samples (A) showed superiority in all growth parameters measured as compared to that of seedlings grown from low yield seed samples (B). Significant difference was shown between the two samples of seed, whereby the calculated difference was about 30% between the two means in weight per gram. Mean values ± S.E were, 11.7±0.28 and 8. 70±0 .15 gram for 100 seed/sample respectively. Other seed parameters were also measured and determined. Vegetative propagation experiments, stem cuttings, in-vitro shoot regeneration, callus initiation / growth and development were conducted. Stem cuttings were taken from trees of different ages and specifications, treated with 10/ 100 and: 000 mg/l 3-indolebutyric acid(IBA). Stem cuttings treated with 100 mg/l IBA showed vigorous growth of shoot, also the number of roots and their lengths were highly significant than with the other concentrations. Rooting percentage was 16.7% for old explant and 10% for younger ones / and successful rooting was obtained during winter and autumn. In-vitro germination, micro propagation and somatic embryogenesis of A. senegal tissues have been also demonstrated. Explant was excised from apical and axillary shoots. Shoot regeneration was obtained with the various concentrations of the growth regulators (l-Naphthalene acetic acid (NAA) and kinetin). The multiplication rate obtained was low (xl and x2) in-spite of the various treatments, and the shoot regenerated showed high mortality rate due to the small number of shoots per explants. Explants of different ages and origins were excised for callus initiation and development. The callus was formed at the basal cut end of the explant with varying intensities and coloration, with the various concentrations of NAA and cytokinin seed. Sequential sub culturing showed a high capability of A. senegal callus tissue for differentiation and development ovulation, pre-embryo, endospermic cells and whole mounts of mature embryos with their shoot and root apices were demonstrated. The results represented In this study should encourage the continuation on investigation on the various aspects leading to enhancing and increasing application of vegetative propagation methods for A. senegal.
URI: http://khartoumspace.uofk.edu/handle/123456789/15456
Date: 2015-06-28


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