University of Khartoum

Antimicrobial Susceptibility of E.coli and Klebsiella spp. Isolated From Leafy Green Vegetables

Antimicrobial Susceptibility of E.coli and Klebsiella spp. Isolated From Leafy Green Vegetables

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Title: Antimicrobial Susceptibility of E.coli and Klebsiella spp. Isolated From Leafy Green Vegetables
Author: AbdElmajed, Ayat Ahmed Mohamed; Alnoor, Elaf Mohammed; Siddig, EtharSuliman; Ahmed, Khadega Osman; Joubara, Sara Jamal
Abstract: Background: Nowadays the spread of antimicrobial resistance particularly multi-drug resistant Escherichia coli and klebsiella species has become major health problem leading to treatment failure. Design: Descriptive cross-sectional study. Objective: This study aimed to determine the antimicrobial susceptibility of Escherichia coli and Klebsiella species isolated from leafy green ready to eat vegetables. Method: 45 vegetable samples were collected from different popular and local markets in Khartoum State, Sudan. All samples were cultured and bacteria were isolated in different culture medium and then they were identified by conventional biochemical tests and API20E strips. Antimicrobial susceptibility testing for E.coli and klebsiella spp. was done using disk diffusion technique according to Clinical Laboratory Standards Institute (CLSI) guidelines. Result: Of 164 isolates, 85.9% Klebsiella spp., 3.6% E.coli and 10.3% other organisms including (Kluyvera spp., Enterobacter cloacae, Serratiaficaria, Serratialiquefaciens, Citrobacterfreundii and Pantoea spp. 2, 4). 24.8% of klebsiella spp. showed resistance to augmentin (amoxicllin-clavulanic acid), 12.2% resistant to cefixime, 3.4% resistant to ciprofloxacin, 0.7% resistant to cotrimoxazole and gentamicin, and all Klebsiella spp. showed no resistance to ceftriaxone and meropenem. E.coli showed high susceptibility rate (100%) to all antimicrobials above. Conclusion: E.coli isolates showed high susceptibility to all antimicrobial agents used in the study while Klebsiella spp. showed low resistance to amoxicillin-clavulanic acid and cefixime and susceptibility to the other antimicrobials used.
URI: http://khartoumspace.uofk.edu/123456789/16719
Date: 2014


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