University of Khartoum

Spectrophotometric Determination of N_ Acetylcysteine and Aminocaproic acid

Spectrophotometric Determination of N_ Acetylcysteine and Aminocaproic acid

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Title: Spectrophotometric Determination of N_ Acetylcysteine and Aminocaproic acid
Author: Elfangary, Sara Ahmed Hassan
Abstract: Simple, rapid, non hazardus, accurate, non expensive and precise spectrophotometric method was developed and optimized for the determination of acetylcysteine( antidote for paracetamol poisoning) and aminocaproic acid(antifibrinolytic drug) in their pure forms and pharmaceutical formulations. The official methods for these drugs were non aqueous titration for acetylcysteine which is hazardous and non accurate or HPLC for aminocaproic acid which is expensive. The study included a color development for the assay of these drugs, investigation of the optimum reaction conditions for the developed color, and the validity of the proposed methods. The study also included the effect of possible interfering substances and traces of other amino acids. Methodology: Acetylcysteine The developed method was applied for analysis of this drug; it utilized 2, 6-dichloroquinone-4-chloroimide (DCQ) reagent which react with Acetylcysteine producing a more UV absorbing complex. The other part utilized ascorbic acid reaction with the primary aliphatic amine (aminocaproic acid). Results: The Acetylcysteine-DCQ complex gave linearity that obeyed Beer"7; law with correlation coefficient of 0.998 in the concentration range 10-50 µg/ml at 438 nm. Aminocaproic acid –ascorbic acid complex gave linearity at 530 nm i.e. obeyed Beer"7; law with correlation coefficient of 0.999 in the concentration range 4-20 µg/ml. These methods were optimized and validated. The precision of the method was determined using three different concentrations of NAC .The results showed a low (SD) varying from 3.055x10-3 to 1x10-3 with relative standard deviation(RSD %) varying from 2.47 to 1.66 % for acetylcysteine concentrations in the range 6.19x10-5 to 3.06x10-4 M. This reflected reproducibility of the method The validity of the method was tested by analyzing samples of acetylcysteine. Under the optimum experimental conditions employed, Beers law was found to be valid over the concentration range [6.1 x 10-5 M – 3.05 x 10-4 M]. The molar absorptivity (=541;) of the complex was 2130.96 L mole -1 cm -1. Beer"7; law was obeyed for aminocaproic acid concentration 4 – 20 µg/ml (3.049 x 10-5 M - 1.52 x 10-4 M). The molar absorptivity (=541;) of the complex was 2492.6 L mole-1 cm-1. The high (=541;) confirmed the sensitivity of the method. The accuracy of the procedure and freedom of interference by the injection excipients was confirmed by the obtained results for recovery testing of added amount of authentic ACA to the injection solution in the ratio of 1:1.The results showed good recovery for the injection ( =108.23±1.45, n = 3). This, together with the good reproducibility reflected the good accuracy and precision of the method. Conclusion: The developed methods were successfully applied in the quantitative assay of commercial capsules and injections, results of the analysis by the developed methods were found to be similar to those obtained by the official methods. The developed methods are simple, rapid and of high accuracy. They recommended for use in routine analysis
URI: http://khartoumspace.uofk.edu/123456789/16745
Date: 2015-10-26


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