University of Khartoum

Effect ofGreen Tea(Camellia sinensis) Extracton Lipid Profile in Rats

Effect ofGreen Tea(Camellia sinensis) Extracton Lipid Profile in Rats

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Title: Effect ofGreen Tea(Camellia sinensis) Extracton Lipid Profile in Rats
Author: Mustafa, Nazik Mohamed Elamin Suliman
Abstract: Introduction:Several studies were carried out with green tea extracts,some evidence suggesting that regular green tea drinkers have lower chances of heart disease and developing certain types of cancer. Green tea contains high concentration of catechin; the major compounds include epigallocatechin-3-gallate (EGCG), epicatechin (EC), and epicatechin-3-gallate (ECG), and epigallocatechin (EGC). EGCG is the most abundant polyphenol present in green tea. Objectives:To evaluate the effect of green tea (Camellia sinensis) extract on lipid profile in rats using Atrovastatin as standard drug. Methods:Healthy male Wistar rats’, weighing 150-300, wasused. They were divided into eight groups each group contains five rats. Group A was the control group which was fed normal diet.Group B fed cholesterol diet during all the weeks. Animals were fed normal diet +1% cholesterol to produce hypercholesterolemia after two weeks for groups C and Dand after four weeks for groups E and F. Group G was concomitantly given the extract with the cholesteroldiet and Group H was given the extract without cholesterol diet for six weeks. Green tea extract was used for treatment at a dose of 1g/kg body weight and Atorvastatin was used as standard drug for the treatment of hyperlipidemia at a dose of 1mg/kg body weight. The blood samples from each rat were collected every two weeks for investigation. Results:Addition of 1% cholesterol to the normal diet for two and four weeks cause significant elevation of total serum cholesterol and LDL-cholesterol level to groups C, D and E,F and a significant decrease in serum HDL- cholesterol levels for group E and F. Administration of green tea extract at a dose of 1g/kg body weight reduced the elevated serum cholesterol and LDL-cholesterol after two weeks of cholesterol diet in group C and after four weeks in group E.HDL-cholesterol showed no significant change in group C and significantly increased in group E after two weeks of the treatment with the extract. Group D and F showed a significant decrease in both serum cholesterol and LDL-cholesterol upon treatment with 1mg/kg of Atorvastatin. HDL-cholesterol showed no significant change in group D and significant increase in group F after twoweeks of the treatment with Atorvastatin. Concomitant administration of green tea extract with cholesterol diet in group G causes no significant change in serum LDL-cholesterol and HDL-cholesterol level. However total cholesterol levels showed a significant increase at the first two weeks and then maintained.Also administration of green tea extract without cholesterol diet in group H lead to significant decrease of total cholesterol at the first two weeks and then maintained. The triglyceride levels showed no significant change in all groups. Histopathological results for groups treated with green tea extract after two and four weeks of cholesterol diet showed very few vaculation in hepatocytes, alsogroups treated with Atorvastatin after two and four weeks of cholesterol diet showed moderate and very few vaculation respectively.Concomitant administration of green tea extract with the cholesterol diet showed almost normal liver that means protection of hepatocytes against the cholesterol effects. Conclusion:Green tea extract in the dose of 1g/kg body weight cause a significant reduction of both the serum cholesterol and LDL-cholesterol levels upon elevation with dietary cholesterol as Atorvastatin does
URI: http://khartoumspace.uofk.edu/123456789/16747
Date: 2015-10-26


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