University of Khartoum

Detection of Coliform Bacteria in Drinking Water in Khartoum State

Detection of Coliform Bacteria in Drinking Water in Khartoum State

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Title: Detection of Coliform Bacteria in Drinking Water in Khartoum State
Author: Haseeb, Hajras Tajalsir Mahmoud
Abstract: The study was carried out to investigate the microbial quality of drinking water in the Great Khartoum. Water samples were collected in duplicate from eighty-four water sources in Khartoum, Bahry and Omdurman cities. Water sources include tap water, public watering stands (Sabeel), house water storage tanks, electric coolers and water treatment stations. Twenty double samples were collected from each source except the water treatment stations from which a pair of double samples was collected before and after water treatment. Samples were divided into two identical groups and analyzed for faecal pollution in the Microbiology laboratories at the Dept. of Microbiology, F. Vet. Med., U of K. and at the Khartoum State Water Corporation Station in El-Mugran. All samples were investigated, as recommended by the WHO, with the Multiple Tube Fermentation Technique (MTF) for total and thermotolerant coliforms and with the Membrane filtration (MF) Technique for total coliforms only. The results were analyzed statistically using SPSS One-Way ANOVA. Total coliforms were detected in 64.3% (54 samples, detected range 2- >1600 cells/100 ml) and in 48% (40 samples, detected range 1-28 CFU/100 ml) of the total water samples when investigated with MTF and MF techniques respectively. The thermotolerant coliforms were detected in 36 samples (43%) with cell count range (2-300 cells/100 ml of water). Detection of thermotolerant coliforms revealed no statistically significant difference (P = 0.520) in samples from the different areas. However, significant difference (P = 0.000005) was observed in the detection of thermotolerant coliforms and their count in water samples from different sources. 50% of the positive samples were detected in Sabeel water (18 samples, detected range 4-300 cells/100 ml), 25% in tank water (9 samples, detected range 2-8 cells/100 ml), 22% in cooler water (8 samples, detected range 2-17 cells/100 ml) and only 2.8% of the positive samples were detected in tap water (1 sample, 4 cells/100 ml). Thermotolerant coliforms were not detected in water station samples after treatment while before treatment they ranged between (17- 26 cell/100 ml). It was concluded from the present study that the microbiological quality of drinking water in the main water stations (after treatment) and within network pipes in Khartoum city met the WHO and SSMO standards at the time of the study, however; it became remarkably faecally polluted in public watering stands (Sabeel) and electric coolers distributed in different private and public places in Khartoum State.
URI: http://khartoumspace.uofk.edu/123456789/16940
Date: 2015-11-10


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