University of Khartoum

Phytochemical Screening and Anti-inflammatory activity of some Plants used in Sudanese Folkloric Medicine

Phytochemical Screening and Anti-inflammatory activity of some Plants used in Sudanese Folkloric Medicine

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Title: Phytochemical Screening and Anti-inflammatory activity of some Plants used in Sudanese Folkloric Medicine
Author: Mohamed, Mona Salish
Abstract: Inflammation is a complex pathophysiological process mediated by a variety of signaling molecules and can be classified as either acute or chronic. Chronic inflammatory diseases remain one of the world’s major health problems. This study aimed to evaluate the anti-inflammatory activity of some plants used in Sudanese folkloric medicine; bioactivity guided fractionation; phytochemical screening of the most active plants. It also aimed to identified, characterized and structure elucidation of the isolated constituents; biological effectiveness of these isolates and to develop High Performance Thin Layer Chromatography (HPTLC) and high resolution Gas Chromatography-Mass Spectroscopy (GC-MS) fingerprint profile of Anti-inflammatory active extract fractions of Tribulus terrestris. Method: Seventeen medicinal plants belonging to different genera and commonly used in Sudanese folkloric medicine as anti-inflammatory remedies, were investigated for their anti-inflammatory activity by using carrageenan induced inflammation in rats’ paws model. Standard Indomethacine was used as (+ve) control and the difference in results was considered significant when P < 0.05. Results: Most of plants extracts (63.9%) showed good impact with inflammatory mediators (78.3% out of this percent represented methanolic extract), 27.8% showed no activity and 8.3% enhanced the carrageenan induced inflammation. The most active plant extracts (Capparis deciduas, Cyperus rotundus and Tribulus terrestris) were further subjected for fractionation by using chloroform, ethyl acetate, n-butanol and water sequentially, and then tested for their anti-inflammatory activity. Bioactivity guided fractionation of Capparis deciduas led to isolation and characterization of three compounds with potent anti-inflammatory activity. Bioactivity guided fractionation of Cyperus rotundus led to isolation and characterization of two compounds; with known anti inflammatory activity. These compounds were isolated by using different chromatographic techniques and their structures were identified by 1D NMR spectrum (C13and 1H-NMR), 2D NMR (COSY, HSQC, HMBC Correlation), X-ray crystallography and GC-MS analysis. HPTLC (High performance thin layer chromatography) and GC-MS studies of Tribulus terrestris revealed the presence of different spots/peaks in different Rf values, corresponding to different compounds. The Suggested structures for those compounds were accomplished using computer search by matching spectra with reference ones in the computer library (NIST and WILLY libraries).This fingerprinting was performed for the first time for this plant. Conclusion: This study validated the traditional uses of the evaluated plants species in treating inflammatory disorders; beside that it will act as a very good data base for these plants. The compounds which were isolated may provide a new lead for more potent analogues and this research represents advanced methodological qualitative analysis of complex matrix such as herbal medicine where only very limited information can be acquisted using traditional techniques.
Description: 191page
URI: http://khartoumspace.uofk.edu/123456789/17000
Date: 2015-11-12


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