University of Khartoum

Transfusion-transmitted Malaria in Khartoum, Sudan: Comparison of Microscopy, Rapid Diagnostic Test and Nested PCR Methods

Transfusion-transmitted Malaria in Khartoum, Sudan: Comparison of Microscopy, Rapid Diagnostic Test and Nested PCR Methods

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Title: Transfusion-transmitted Malaria in Khartoum, Sudan: Comparison of Microscopy, Rapid Diagnostic Test and Nested PCR Methods
Author: Ali, Raga eltahir mustafa; Abdel Hamid, Muzamil M.; A. Amine, Ahmed A.
Abstract: Malaria can be efficiently transmitted by the transfusion of cellular blood components. It is undoubtedly responsible for the majority of transfusion transmitted diseases in the world. The World Health Organisation recommends that donated blood should be tested for malaria ‘‘where appropriate and possible’’, but there is currently no method for screening blood for low-level parasitaemia that is sensitive, practical and affordable for use by transfusion services in endemic countries. This study aimed to determine the prevalence of malaria parasitaemia in blood transfusion donors in Khartoum, Sudan; and to evaluate three diagnostic tests: microscopy, rapid diagnostic test and molecular technique, for the detection of malaria parasites. Following informed consent Two hundred blood donors were enrolled. Two ml of EDTA anticoagulated blood was collected from each donor for Conventional thin and thick blood smears, an immunochromatographic test, and molecular technique. Malaria parasites were detected in 6% by microscopic examination, 6.5 % by immunochromatographic test and 18.5 by molecular technique. In conclusion, we determine high prevalence of malaria parasitaemia among blood transfusion donors in Khartoum state, Sudan, with variable detection sensitivity between the three different techniques. Our finding highlighted the urgent need to develop an effective strategy for the prevention of transfusion-transmitted malaria in Sudan.
URI: http://khartoumspace.uofk.edu/123456789/17016
Date: 2015-11-12


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