University of Khartoum

An Investigation of Crk Prote in Kinases of Leishmania and the Assessment of Their Potential as Drug Targets

An Investigation of Crk Prote in Kinases of Leishmania and the Assessment of Their Potential as Drug Targets

Show full item record

Title: An Investigation of Crk Prote in Kinases of Leishmania and the Assessment of Their Potential as Drug Targets
Author: Ibrahim, Muntaser E.; Ali, Nahla; Aradaib, Imadeldin E.
Abstract: Cyclin-dependent kinases (CDKs), exemplified by cdc2, are key regulators of the eukaryotic cell cycle [1]. A number of cdc2-related kinase genes have been isolated from trypanosomatids [2-6]. The present study was designed to identify and analyze cdc2-related protein kinases (CRKs), investigate their regulation mechanisms, and thus their role in regulating the cell cycle of the protozoa Leishmania donovani, the causative agent for Kala-azar and Leishmania mexicana; the causative agent for New world cutaneous leishmaniasis. To achieve this aim, CRK3 protein kinase gene was isolated from a Sudanese strain of L. donovani, designated LdCRK3. It encodes a protein of 311 amino acids with 99.7 % identity with the L. mexicana CRK3 and 49.4% identity with human Hscdc2. Southern blot analysis showed that LdCRK3 is single copy, consistent with the genomic organization of all the so-far identified trypanosomatid protein kinases. The sequence of L. donovani CRK3 has been deposited in GenBankTM under the access number AJ426472.
URI: http://khartoumspace.uofk.edu/123456789/17109
Date: 2015-11-15


Files in this item

Files Size Format View

This item appears in the following Collection(s)

Show full item record

Share

Search DSpace


Browse

My Account