Title:
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Anti - Glucose Regulated Protein 78 (GRP78) antibody responses in Alum/ALM plus BCG vaccinee, visceral leishmaniasis, and Post Kala- azar Dermal leishmaniasis patients |
Author:
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; Musa, Ahmed M.; Elhassan, Ahmed M.; Khalil, Eltahir Awad G.; and, etal
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Abstract:
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Introduction: The Glucose Regulated Protein 78 (GRP78) of Leishmania donovani is considered
to be one of the potential Leishmania vaccine candidates.
Patients and methods: Using Enzyme-Linked Immunosorbent Assay (ELISA), we measured IgG
antibody responses to GRP78 in 39 healthy Sudanese volunteers vaccinated with Leishmania
Alum/ALM + BCG vaccine, 29 patients with visceral leishmaniasis (VL), and 26 patients with post
kala-azar dermal leishmaniasis (PKDL).
Results: There was, no significant statistical difference in plasma levels of GRP78 antibodies in
immunized and control group (P=0.37). Furthermore, no significant statistical difference in the levels of GRP78
antibodies in the pre and post vaccination plasma samples (P=0.60). Plasma IgG levels to GRP78 was significantly
higher in visceral leishmaniasis and PKDL patients compared with control group (P=0.00).
Conclusion: This study concludes that Alum/ALM vaccine does not induce a Th2 type of immune response. It also
demonstrated clearly that VL and PKDL are associated with elevation of anti-GRP 78 antibodies and that GRP78
ELISA can be used to confirm diagnosis of Leishmania infections based on the clinical presentation. |
URI:
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http://khartoumspace.uofk.edu/123456789/17123
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Date:
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2015-11-16 |