University of Khartoum

Molecular Characterization of Sudanese and Southern Sudanese Chicken Breeds Using mtDNA D-Loop

Molecular Characterization of Sudanese and Southern Sudanese Chicken Breeds Using mtDNA D-Loop

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Title: Molecular Characterization of Sudanese and Southern Sudanese Chicken Breeds Using mtDNA D-Loop
Author: Wani, Charles E.; Ibrahim, Muntaser E.; Musa, Ahmed M.
Abstract: The objective of this study was to assess the genetic relationships and diversity and to estimate the amount of gene flow among the five chicken populations from Sudan and South Sudan and commercial strain of egg line White Leghorn chickens. The chicken populations were genotyped usingmtDNA D-loop as amolecularmarker. PCR product of themtDNA D-loop segment was 600 bp and 14 haplotypes were identified. The neighbor-joining phylogenetic tree indicated that the indigenous Sudanese chickens can be grouped into two clades, IV and IIIa only. Median joining networks analysis showed that haplotype LBB49 has the highest frequency. The hierarchal analysis ofmolecular variance (AMOVA) showed that genetic variation within the population was 88.6% and the differentiation among the population was 11.4%. When the populations was redefined into two geographical zones, rich and poor Savanna, the results were fractioned into three genetic variations: between individuals within population 95.5%, between populations within the group 0.75%, and genetic variation between groups 3.75%.The pair wise 𝐹st showed high genetic difference between Betwil populations and the rest with 𝐹st ranging from 0.1492 to 0.2447. We found that there is large number of gene exchanges within the Sudanese indigenous chicken (Nm = 4.622).
URI: http://khartoumspace.uofk.edu/123456789/17143
Date: 2015-11-16


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