University of Khartoum

Isolation of Streptomyces from Soil, Screening for Antibiotic Activity and Effect of UV Mutagenesis on Antibiotic Activity of Isolates.

Isolation of Streptomyces from Soil, Screening for Antibiotic Activity and Effect of UV Mutagenesis on Antibiotic Activity of Isolates.

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Title: Isolation of Streptomyces from Soil, Screening for Antibiotic Activity and Effect of UV Mutagenesis on Antibiotic Activity of Isolates.
Author: Abbo, Sarra Elsayed Ali Elsayed
Abstract: Ten Streptomyces sp. Strains were isolated from different locations in the Sudan. The isolates were identified as Streptomyces sp by microscopical, biochemical and cultural charcatersistics tests in accordance with cited literature on the subject. The ten isolates were characterized for their inhibitory antibiotic effect on seven different types of reference bacterial strains. The test strains were Bacillus subtilis, Psedomonas aeruginosa, Klebsiella pneumonia, Staphllococcus sp., Escherichia coli and Staphylococcus aureus. Streptomyces isolates S7 and S10 displayed more general inhibitory antibiotic effect than all of the rest Streptomyces isolates. Isolate S7 demonstrated an inhibtory zones of 4.0, 3.0 and 1.0 mm against S. aureus, E.coli and Streptococcus sp. respectively. The rest of the isolates demonstrated an inhibitory antibiotic effect of 2.0 to 0.1 mm or no inhibitory effect at all. UV mutagenesis induced a general negative effect on antibiotic activity against the tested strains for isolates S1 to S5 however, a positive effect of the mutagenic activity was observed for isolate S7. This isolate demonstrated an inhibition zone of 7.0 mm after mutagenesis for S. aureus instead 4.0mm and 5.0 mm for E.coli instead 3.0. A marked positive inhibitory effect was observed for Streptomyces isolate S10. The inhibitory zone increased for E.coli from 4.0 to 6.0 and from 3.0 to 11.0 for Streptococcus sp. However, no effect was observed after mutagenesis for the rest of the test strains. These results is encouraging with respect to the potential of UV, or other, mutagenic treatment of characterized Streptomyces isolates for enhancement of antibiotic production. We recommend that this work to be extended for inclusion of more isolates and refined chemical characterization and structure of the active antibiotic inhibiting agent as well as more rigorous programs for induction of mutagenesis of isolates of a good potential with the aim of enhancement of their antibiotic productivity.
Description: 52page
URI: http://khartoumspace.uofk.edu/123456789/17187
Date: 2015-11-17


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