University of Khartoum

Application of molecular biological techniques for detection of epizootic hemorrhagic disease virus (EHDV-3 18) recovered from a sentinel calf in central Sudan

Application of molecular biological techniques for detection of epizootic hemorrhagic disease virus (EHDV-3 18) recovered from a sentinel calf in central Sudan

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Title: Application of molecular biological techniques for detection of epizootic hemorrhagic disease virus (EHDV-3 18) recovered from a sentinel calf in central Sudan
Author: Mukhtar, Moawia M.; Mohammed, M.E.H.
Abstract: Epizootic hemorrhagic disease virus (EHDV), isolate 318 (EHDV-318), an untyped virus recovered from a sentinel calf herd at the Khartoum University farm in central Sudan, was characterized using molecular biological techniques. With dot blot hybridization technique, a cDNA probe derived from genome segment 6 of EHDV-2 (Alberta strain) hybridized with RNA from EHDV-318. Application of serogroup-specific EHDV polymerase chain reaction (PCR) to EHDV-3] 8 RNA resulted in specific amplification of a 387 by PCR product. Amplification product was visualized on ethidium bromide-stained agarose gel. Specificity of the PCR products was confirmed by chemiluminescent hybridization with a non-radiolabelled internal probe. No amplification product or hybridization signal was detected when the serotype-specific EHDV-1 or EHDV-2 PCR-based assays were applied to RNA from EHDV-318. The scientific data presented in this study indicated that cDNA probes and serogroup-specific PCR-based assay can be used to classify the virus as a member of EHDV serogroup, and as serotypically distinct from EHDV-1 and EHDV-2.
URI: http://khartoumspace.uofk.edu/123456789/17284
Date: 2015-11-25


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