University of Khartoum

Leishmania Resistant to Sodium Stibogluconate: drug-Associated Macrophage-Dependent Killing

Leishmania Resistant to Sodium Stibogluconate: drug-Associated Macrophage-Dependent Killing

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Title: Leishmania Resistant to Sodium Stibogluconate: drug-Associated Macrophage-Dependent Killing
Author: Elhassan, Ahmed M; Ibrahim, Muntaser E.
Abstract: A total of 17Leishmania isolates, 6 of them isolated from antimony-resistant patients, were collected in the Sudan and tested for their sensitivity to sodium stibogluconate (Pentostam) as promastigotes. Six of those isolates were tested as amastigotes infecting a murine macrophage cell line. The results indicated that the conventional promastigote screening assay did not correlate with the clinical picture, whereas the amastigote/macrophage system produced results that pertained to the in vivo responses to the drug. A laboratory-generated resistant strain ofL. major was adapted to grow at a high concentration of Pentostam (1000 μg/ml) as promastigotes but was quite sensitive to the drug at much lower concentrations in the amastigote/(macrophage system (20μg/ml), thus suggesting that Pentostam's inhibitory action is mediated through the macrophage rather than through a direct toxic effect exerted on the parasite
URI: http://khartoumspace.uofk.edu/123456789/17339
Date: 2015-11-29


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