University of Khartoum

Evaluation of the Polymerase Chain Reaction in the Diagnosis of Cutaneous Leishmaniasis due to Leishmania major: A comparison with direct Microscopy of Smears and Sections from Lesions

Evaluation of the Polymerase Chain Reaction in the Diagnosis of Cutaneous Leishmaniasis due to Leishmania major: A comparison with direct Microscopy of Smears and Sections from Lesions

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Title: Evaluation of the Polymerase Chain Reaction in the Diagnosis of Cutaneous Leishmaniasis due to Leishmania major: A comparison with direct Microscopy of Smears and Sections from Lesions
Author: Elhassan, Ahmed M
Abstract: We have compared the sensitivity of the polymerase chain reaction (PCR) as a diagnostic tool against conventional microscopical diagnostic techniques in patients with cutaneous leishmaniasis from the Sudan. Twenty-eight patients were diagnosed according to clinical criteria followed by microscopical examination of histological sections and slit or impression smears. The PCR had a sensitivity of 86% when used alone, and 93% when combined with Southern blotting. In contrast, microscopy of histological sections had a sensitivity of 76% and slit and impression smears of only 55% and 48%, respectively. The PCR should be considered as a valuable and sensitive diagnostic tool in the diagnosis of cutaneous leishmaniasis; it has the added advantage of identification of the species of Leishmania causing the lesion.
URI: http://khartoumspace.uofk.edu/123456789/17351
Date: 2015-11-29


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