Title:
|
Immunogenicity and Immune Modulatory Effects of in Silico Predicted L. Donovani Candidate Peptide Vaccines |
Author:
|
Khalil, Eltahir Awad G.; Musa, Ahmed M.; Elhassan, Ahmed M.
|
Abstract:
|
Visceral leishmaniasis (VL) is a serious parasitic disease for which control measures are limited and drug resistance is
increasing. First and second generation vaccine candidates have not been successful. The goal of the present study was
to select possibly immunogenic L. donovani donovani GP63 peptides using immunoinformatics tools and to test their
immunogenicity in vitro. The amino acid sequence of L. donovani donovani GP63 [GenBank accession: ACT31401] was
screened using the EpiMatrix algorithm for putative T cell epitopes that would bind to the most common HLA class II
alleles (DRB1*1101 and DRB1*0804) among at–risk populations. Four T cell epitopes were selected from nine potential
candidates. Stimulation of whole blood from healthy volunteers using the peptides separately produced mean IFN-γ and
IL-4 levels that were not significantly different from negative controls, while the pooled peptides produced a moderate
IFN-γ increase in some volunteers. However, mean IL-10 levels were significantly reduced for all individuals compared with
controls. The immunogenicity of these epitopes may be harnessed most effectively in a vaccine delivered in combination
with immune-modulating adjuvants. |
URI:
|
http://khartoumspace.uofk.edu/123456789/17372
|
Date:
|
2015-12-01 |