University of Khartoum

Detection And Identification Of Leishmania Species Within Naturally Infected Sand Flies In The Andean Areas Of Ecuador By A Polymerase Chain Reaction

Detection And Identification Of Leishmania Species Within Naturally Infected Sand Flies In The Andean Areas Of Ecuador By A Polymerase Chain Reaction

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Title: Detection And Identification Of Leishmania Species Within Naturally Infected Sand Flies In The Andean Areas Of Ecuador By A Polymerase Chain Reaction
Author: Kato, Hirotomo
Abstract: The surveillance of prevalent Leishmania and sand fly species in endemic areas is important for prediction of the risk and expansion of leishmaniasis. In this study, we developed a polymerase chain reaction (PCR)−based method for detection of Leishmania minicircle DNA within individual sand flies. Using this method, we detected minicircle DNA in 6 (3.3%) of 183 sand flies, while 5 (3.5%) of 143 were positive for Leishmania promastigotes in the same areas by microscopic examination. The species were identified as Leishmania (Leishmania) mexicana by nucleotide sequencing of the cytochrome b gene. Additionally, all the Leishmania-positive sand flies were identified as Lutzomyia ayacuchensis by the restriction enzyme digestion of the PCR-amplified 18S ribosomal RNA gene fragments. Since this combined method is relatively easy and can process a large number of samples, it will be a powerful tool for the rapid identification of prevalent sand fly and Leishmania species as well as monitoring the infection rate in sand fly populations in endemic areas.
URI: http://khartoumspace.uofk.edu/123456789/17380
Date: 2015-12-01


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