Title:
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Diagnosis of visceral leishmaniasis by the polymerase chain reaction using blood, bone marrow and lymph node samples from patients from the Sudan |
Author:
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Elhassan, Ahmed M.; Khalil, Eltahir Awad G.
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Abstract:
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We have evaluated the sensitivity of the polymerase chain reaction (PCR) as a diagnostic
tool for Leishmania donouani using blood, bone marrow and lymph node samples from
Sudanese patients with a confirmed infection. Forty patients were diagnosed by microscopic
examination of bone marrow or lymph node samples. The PCR was able to detect parasite
DNA in 3 7 out of 40 blood samples. In bone marrow and lymph node samples, the PCR
was able to detect parasite DNA in all 7 and 6 samples, respectively. We suggest that the
PCR should be considered as a valuable and sensitive tool for the diagnosis of 1.. donouani
infection. However, if PCR diagnosis is to supplement or even replace microscopic diagnosis
in developing countries, a large number of patients with no apparent signs of infection and
patients with other diseases have to he tested in order to evaluate its true potential. |
Description:
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5 Pages |
URI:
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http://khartoumspace.uofk.edu/123456789/17382
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Date:
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2015-12-01 |