University of Khartoum

Phytochemical Composition and Bioassay of Oleogum Resins of Boswellia papyrifera (Tarak tarak), grown in Some Parts of the Sudan

Phytochemical Composition and Bioassay of Oleogum Resins of Boswellia papyrifera (Tarak tarak), grown in Some Parts of the Sudan

Show full item record

Title: Phytochemical Composition and Bioassay of Oleogum Resins of Boswellia papyrifera (Tarak tarak), grown in Some Parts of the Sudan
Author: Mustafa, Ghada Ibrahim
Abstract: Three samples of oleogum resins of Boswellia papyrifera grown in some parts of the Sudan were subjected to screening with the objective of evaluating their phytochemical constituents, antimicrobial and antioxidant activities as well as testing their cytotoxicity potentials. The essential oil content of the three samples ranged between 2.14% and 3.33%. The acid value varied from 4.36 to 9.55. The three essential oils showed significantly similar saponification, ester and refractive indices. Gas chromatography/Mass spectrometry (GC/MS) analysis revealed that the major compound of the essential oils was octyl acetate, 46.50%, 39.31% and 35.31% for Kordofan, Damazine and Nagawa, respectively. The main components in the petroleum ether extracts were sequentially naphtho [1, 2-b] furan-2-one (24.23%), oleic acid (22.39%) and octadecanoic acid (20.45%), whereas the principal chemical compounds in the methanolic extracts were lycopene (13.78%), oleic acid (28.60%) and benzoic acid (26.90%). The dominant molecule in the acid fractions of both Kordofan and Damazine samples was hexadecanoic acid (16.46% and 23.33%, respectively), while that from Nagawa olibanum was oleic acid (15.34%). The essential oils of the three samples showed high antibacterial activity against all tested bacteria (Basillus. subtilis, Staphylococcus. aureus, Echercshia. coli, Pseudomonas. aeruginosa and Salmonella. xii typhi) with minimum inhibition concentration (MIC) of 5 - 10 μg/ ml. In addition, they demonstrated antifungal activity against Aspergillus niger, A. flavus and Candida albicans with MIC of 5 μg/ ml. Petroleum ether extracts and acid fractions derived from the three samples showed high antibacterial activity against S. aureus, E. coli and B. subtilis. Chloroform extracts and non-acid fractions of both Kordofan and Damazine frankincense suppressed the growth of B. subtilis, E. coli and P. aeruginosa. S. typhi showed resistance to all used materials except the non-acid fraction from Kordofan frankincense. Furthermore, all tested bacteria (excluding B. subtilis) and fungi (except Candida albicans) showed resistance to the methanolic and water extracts. All examined materials showed antioxidant activity towards DPPH (2, 2-diphenyl-1-picrylhydrazyl.) radical in dose dependent manner. The essential oils of the three samples showed the highest antioxidant activities (41.76% – 51.38%) and considerable iron chelating abilities. Petroleum ether extracts gave the highest iron chelating potentialities (90.05% – 93.15%). Investigation using the brine shrimp assay revealed a dosedependent response in mortality. Most of the examined materials possessed high cytotoxicity with LD50 values of less than 20 μg /ml. The essential oils and various extracts from Sudanese B. papyrifera can be of potential use as antimicrobial agents and natural antioxidants and to predict anti-carcinogenic activity.
Description: 199page
URI: http://khartoumspace.uofk.edu/handle/123456789/17412
Date: 2015-12-03


Files in this item

Files Size Format View

This item appears in the following Collection(s)

Show full item record

Share

Search DSpace


Browse

My Account