University of Khartoum

Molecular genetic characterization of the Growth Hormone gene ( GH1 ) in Sudanese Kenana and Butana cattle breeds

Molecular genetic characterization of the Growth Hormone gene ( GH1 ) in Sudanese Kenana and Butana cattle breeds

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Title: Molecular genetic characterization of the Growth Hormone gene ( GH1 ) in Sudanese Kenana and Butana cattle breeds
Author: Musa, L.M-A.; Nour, I.A.; Brockmann, G.A
Abstract: The aim of the present study was the identification of allelic va riants of the bovine growth hormone 1 ( GH1 ) gene in Sudanese Kenana and Butana cattle. A 2080 bp fragment spanning the region between 146 bp upstream of the first exon and 140 bp downstream of the last exon were sequenced in Kenana and Butana cattle. The sequence comparison of Kenana and Butana GH1 sequences with the reference GenBank sequen ce (M57764) descending from Holstein Friesian identified 10 and 12 single nucleotide polymorphi sms (SNPs), respectively. Three novel SNPs were identified (g.1304C>T, g.2346C>T, and g.2537G>T ) (positions are given relative to the GenBank sequence M57764). A PCR-RFLP method was used to access the allele frequency distribution for the SNPs g.1059C>T ( Msp I restriction, silent substitution in exon 2), g.1547C>T ( Msp I restriction in intron 3), and g.2141C>G ( Alu I restriction, Leu/Val substitution in exon 5) in 61 and 54 animals of the Kenana and Butana breeds, respectively. Both breeds were found to be carrier of g.1059T allele with an allelic frequency of 0.35 for Kenana and 0.24 for Butana, whereas g.2141C allele was found to be fi xed. The allele frequency of g.1547T ( Msp I (-)) was highly significant (p<0.01) in Kenana (0.77) comp ared to Butana (0.59) cattle. The identified SNPs in Kenana and Butana cattle breeds could be used as genetic markers in different genetic studies
URI: http://khartoumspace.uofk.edu/handle/123456789/17516
Date: 2015-12-10


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