University of Khartoum

Esitmation of rumen gas volume by dilution technique in sheep given two silages at different levels of feeding

Esitmation of rumen gas volume by dilution technique in sheep given two silages at different levels of feeding

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Title: Esitmation of rumen gas volume by dilution technique in sheep given two silages at different levels of feeding
Author: Fadel El-Seed, Abdel Nasir M. A.; Kamel, Hossam E. M.
Abstract: The gas dilution technique was used to evaluate the possibili ty of estimating the volume of gaseous phase in the rumen from its composition in sheep given rice whole crop silage (RWS) or dent corn silage (DCS) at a level of maintenance (M) or 2 M , and in the course of fasting. The rumen gas composition was determined at 2 and 7.5 h after morning feeding. Nitrogen gas was injec ted by using an airtight syringe into the rumen immediately after collect ing the rumen gas sample as a c ontrol. Then rumen gas samples were collected at 5, 10, 20, 40 and 60 min. afte r injection. Dry-matter intakes were 42 g/kg 0.75 and 57 g/kg 0.75 for DCS, and 36 g/kg 0.75 and 59 g/kg 0.75 for RWS, at 1 M and 2 M levels, respectiv ely. Animals ingested both silages about 20% less than e xpected at 2 M level. The rumen gas composition did not differ significantly be tween 2 h and 7.5 h after feeding except for N 2 . Content of CO 2 in gas composition was significantly higher at 2 M level than at 1 M (p<0.05) for both RWS and DCS, whereas CH 4 showed no significant difference between feeding levels. At both feeding levels, CO 2 showed a higher (p<0.05) percentage in DCS than RWS. A dilution technique by using N 2 injection is not appropriate for the determination of gas production in vivo , unless the rate of rumen gas turnover is considered. Changes in composition at fasting indicate that the rumen fermentation may reach the lowest level after 72 h fasting for sheep given silage as their sole diet.
URI: http://khartoumspace.uofk.edu/handle/123456789/17607
Date: 2015-12-14


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