University of Khartoum

Potassium Efficiency of Different Crops Grown on a Sandy Soil under Controlled Conditions

Potassium Efficiency of Different Crops Grown on a Sandy Soil under Controlled Conditions

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dc.contributor.author Claassen, Norbert
dc.contributor.author El Dessougi, Hanadi I.
dc.date 2010
dc.date.accessioned 2015-12-20T11:49:04Z
dc.date.available 2015-12-20T11:49:04Z
dc.date.issued 2015-12-20
dc.date.submitted 2015
dc.identifier.uri http://khartoumspace.uofk.edu/handle/123456789/17892
dc.description.abstract Kluyveromyces marxianus DMKU3-1042 is a thermotolerant yeast strain suitable for high-temperature ethanol fermentation and genetic engineering with linear DNA. We have developed a highly efficient random gene integration method with a frequency that exceeds 2.5 × 106 transformants/μg linear DNA, a figure comparable to what is observed with autonomously replicating plasmid transformation in Saccharomyces cerevisiae. To establish the mechanism of random integration in DMKU3-1042, we identified and deleted the K. marxianus KU70 gene, which is known to be involved in the non-homologous end-joining (NHEJ) pathway. In yeast lacking KU70, highfrequency non-homologous gene integration was abolished and the Kmku70 mutants showed 82–95% homologous gene targeting efficiencies using homologous sequences of 40–1000 bp. These results indicate that the highly efficient NHEJ pathway can be utilized with random gene disruption techniques such as transposon mutagenesis and plasmid-free gene manipulations in K. marxianus. Copyright  2009 John Wiley & Sons, Ltd. en_US
dc.publisher university of khartoum en_US
dc.subject Nutrient efficiency; potassium; root density; influx en_US
dc.title Potassium Efficiency of Different Crops Grown on a Sandy Soil under Controlled Conditions en_US
dc.type Publication en_US
dc.Faculty Agriculture en_US

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