University of Khartoum

Phenotypic and Molecular Characterization of Kenana, Butana and Erashy Sudanese cattle

Phenotypic and Molecular Characterization of Kenana, Butana and Erashy Sudanese cattle

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Title: Phenotypic and Molecular Characterization of Kenana, Butana and Erashy Sudanese cattle
Author: Osman, Nawal Nour Elda-im Omer
Abstract: Objective: To identify DNA polymorphisms which are related to productive (GH-Leptin gene ) and reproductive (FSHR-LHR gene ) performance . Abstract:Characterization data were obtained on Kenana, Butana and Erashy cattle using a structured questionnaire, interviews and repeated field visits in the northern central and eastern parts of the Sudan.Asample of 230 animals was used to obtain morphometric measurements. Kenana cattle studied were white (64.5%) while Butana were mostly red (71.1%). However; Erashy cattle coat colours were 39.2% white and 38.5% red. The average adult body weights of Kenana, Butana and Erashy were 272.55± 109.28, 282.12±65.6 and 247.46 ±74.90 kg, respectively. Estimates of body lengths of the three breeds were 154.83 ±25.09, 162.4 ±26.93 and 161.2 ±23.82 cm, respectively. Average daily milk production and lactation length of Kenana cattle were 13.4 ± 6.0 pounds and 4.3± 3.1 months, respectively. The corresponding values for Butana cattle were 18.19±5.64 pounds and 4.00±6.70 months, while those for Erashy were 15.35 ± 5.93 pounds and l 5.75 ± 6.71 months. The phenotypic, production and reproduction characterization of the three ecotypes showed significant differences between them. However, these results had to be confirmed by a genetic study at the DNA level. In the second part of the study the genotypic and allelic frequencies of two polymorphisms located in the receptors of the Follicle Stimulating Hormone (FSHR) andreceptors of Luteinizing Hormone (LHR) were estimated. One hundred and sixteen blood samples were collected from Kenana, Butana and Erashy cattle. The studied samples included 32, 34 and 50 cows from each of the three breeds, respectively. The DNA was extracted following standard methods. The purified DNA was subjected to PCR-RFLP techniques to identify polymorphisms of the FSHR and LHR genes in the three Sudanese cattle ecotypes. The amplified fragments of FSHR (306 bp) were digested with restriction enzymes ALuresulting in 243 and 63 bp fragments. At exon 10 in the FSHR gene, all genotyped cows were homozygous for AA genotype. The restriction endonuclease Hhal allowed the identification of three genotypes of the LHR gene at exon 11 among the different ecotypes: TT, CT and CC genotypes. The observed genotypic frequencies for LHR gene in Kenana were 33.3% TT, 41.7% CT and 25% CC. In Butana cattle the frequencies were 18.7% TT, 50% CT and 31.3% CC. In Erashy the frequencies were 33.3% TT, 50% CT and 16.7% CC. Another one hundred and fourteen blood samples were collected from Kenana (32), Butana (32) and Erashy (50) from unrelated animals. DNA was extracted following standard methods. The PCR-RFLP technique was used to screen for DNA polymorphisms of the Growth Hormone (GH) and Leptin (Lep) genes.The ALUI digestion of the 211 bp polymerase chain reaction (PCR) products in the GH gene at exon 5 produced two alleles, namely (L) and (V). In the GH gene, three genotypes were identified in this study. The frequency of LL genotypes in Kenana, was 100%. In Butana, the frequencies of LL and VV were 75% and 25%, respectively. The frequencies of LL and LV in Erashy,were 90% and 10%, respectively. In the leptin gene, the digestion of PCR products between exon2 and intron2 using Sau3Al enzyme revealed the presence of three genotypes: The frequencies of the AA and BB genotypes in Kenana were 96.86% and 3.14%, respectively. The frequencies of AA and AB genotypes were 96.86%, 3.14%, and 80%, 20%, in Butana and Erashy respectively. The study concluded that the three ecotypes are probably distinct and that they should be conserved separately as a valuable genetic resource. The information about genetic variability and the genetic differences within and between populations of indigenous cattleshould be used to develop new selection or introgression strategies for breed improvement and conservation progammes and to define and conserve new sources of genetic variation for future generations
URI: http://khartoumspace.uofk.edu/handle/123456789/17941
Date: 2015-12-22


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