University of Khartoum

Employment of Oligodeoxynucleotide plus Interleukin-2 Improves Cytogenetic Analysis in SplenicMarginal Zone Lymphoma

Employment of Oligodeoxynucleotide plus Interleukin-2 Improves Cytogenetic Analysis in SplenicMarginal Zone Lymphoma

Show simple item record

dc.contributor.author Bardi, Antonella
dc.contributor.author Cavazzini, Francesco
dc.contributor.author Rigolin, GianMatteo
dc.contributor.author Tammiso, Elisa
dc.contributor.author Volta, Eleonora
dc.contributor.author Pezzolo, Elisa
dc.contributor.author Formigaro, Luca
dc.contributor.author Sofritti, Olga
dc.contributor.author Daghia, Giulia
dc.contributor.author Ambrosio, Cristina
dc.contributor.author Rizzotto, Lara
dc.contributor.author Abass, Awad E.
dc.contributor.author D’Auria, Fiorella
dc.contributor.author Musto, Pellegrino
dc.contributor.author Cuneo, Antonio
dc.date 2011
dc.date.accessioned 2016-01-04T12:16:18Z
dc.date.available 2016-01-04T12:16:18Z
dc.date.issued 2016-01-04
dc.date.submitted 2016
dc.identifier.uri http://khartoumspace.uofk.edu/handle/123456789/18191
dc.description.abstract To compare the efficiency of novel mitogenic agents and traditional mitosis inductors, 18 patients with splenic marginal zone lymphoma (SMZL) were studied. Three cultures using oligodeoxynucleotide (ODN) plus interleukin-2 (IL-2), or TPA, or LPS were setup in each patient. Seventeen/18 cases with ODN + IL2 had moderate/good proliferation (94,4%) as compared with 10/18 cases with TPA and LPS (55%) (P = .015); 14/18 (77, 7%) cases with ODN + IL2 had sufficient good quality of banding as compared with 8/18 cases (44, 4%) with TPA and LPS. The karyotype could be defined from ODN+ IL2-stimulated cultures in all 18 patients, 14 of whom (77,7%) had a cytogenetic aberration, whereas clonal aberrations could be documented in 9 and in 3 cases by stimulation with LPS and TPA, respectively. Recurrent chromosome aberrations in our series were represented by aberrations of chromosome 14q in 5 patients, by trisomy 12 and 7q deletion in 4 cases each, and by abnormalities involving 11q and 13q in two cases each. These findings show that stimulation with ODN + IL2 offers more mitotic figures of better quality and results in an increased rate of clonal aberrations in SMZL, making this method ideal for prospective studies aiming at the definition of the prognostic impact of cytogenetic aberrations in this disorder. en_US
dc.language.iso en en_US
dc.publisher Journal of Biomedicine and Biotechnology en_US
dc.subject SplenicMarginal Zone Lymphoma en_US
dc.subject Oligodeoxynucleotide en_US
dc.title Employment of Oligodeoxynucleotide plus Interleukin-2 Improves Cytogenetic Analysis in SplenicMarginal Zone Lymphoma en_US
dc.type Publication en_US
dc.Faculty Medical Laboratory Sciences en_US

Files in this item

Files Size Format View

This item appears in the following Collection(s)

Show simple item record

Share

Search DSpace


Browse

My Account