University of Khartoum

Molecular Detection and Characterization of Human Papilloma Virus in Children with Focal Epithelial Hyperplasia in Khartoum State

Molecular Detection and Characterization of Human Papilloma Virus in Children with Focal Epithelial Hyperplasia in Khartoum State

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Title: Molecular Detection and Characterization of Human Papilloma Virus in Children with Focal Epithelial Hyperplasia in Khartoum State
Author: Agarof, Samira Munir Bolis
Abstract: Background: Focal epithelial hyperplasia (FEH) is a common disease which appears in the form of symptomless benign oral cavity lesions caused by specific human papilloma virus (HPV) genotypes. It is seen worldwide specially among children and mainly in females than males. This study aimed to detect HPV and determine the genotypes assocciated with FEH in childern (three to fourteen years old). The PCR findings were compared to the Pap. stain results. Methods: This descriptive, analytical study was conducted at the Faculty of Dentistry, University of Khartoum and Khartoum Dental Teaching Hospital, Khartoum State, during the period from February 2012 to August 2014. Fourty seven oral biopsies and smears were taken from children (3-14 years old), who attended the pediatric dental clinics and were clinically diagnosed as FEH cases. DNA was extracted from the biopsies using a special tissue DNA extraction kit then polymerase chain reaction (PCR) was performed. Pap. stain was done for the oral smears. Results: Eleven samples (23.4%) were positive for the low risk genotypes 6/11, twenty samples (42.6%) were positive for genotypes 13/32 and twenty seven samples (57.4%) were positive for the high risk genotypes 16/31/33/35/45/52/58 and 66. Genotypes 18/39/56/59 were not detected (0%). Twenty seven samples (57.4%) were positive for more than one genotype. The most frequent genotypes which were detected were HPV 32 (16 samples were positive, 34%) followed by HPV 16 (12 samples were positive, 25.5%). HPV genotypes 31, 33, 35, 52, 58 and 66 were detected using the PCR technique in this study although they were not mentioned in the studies conducted in FEH at the level of the literature review. For the oral smears Papanicolaou (Pap.) stain was performed and twenty nine samples (80.5%) for PCR showed koilocytosis and mitosoid cells. Pap. Stain's sensitivity was 78.4% and 60% specific. Conclusion: The frequency and percentage of HPV genotypes assocciated with FEH was (2.1% – 34%). The percentage of the positive Pap. smears was 76.6%. There was a significant difference between PCR and Pap. Stain. The PCR was the gold (standard) method for diagnosis of FEH cases compared with the Pap stain.
Description: 64page
URI: http://khartoumspace.uofk.edu/handle/123456789/18390
Date: 2016-01-11


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