University of Khartoum

Karyotype Analysis of Acacia Seyal Var Seyal and Var Fistula Natural Population, Gadarif - Sudan

Karyotype Analysis of Acacia Seyal Var Seyal and Var Fistula Natural Population, Gadarif - Sudan

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Title: Karyotype Analysis of Acacia Seyal Var Seyal and Var Fistula Natural Population, Gadarif - Sudan
Author: Khojali, Safaa Salah Aldien Mubarak
Abstract: The objective of this study was to karyotype Acacia seyal var. seyal and var. fistula trees in terms of chromosomes number, length and centromere position, and to study the relationship between the two varieties. Bulk seeds were collected from natural stands of var. seyal and var. fistula in Alrawashda Forest, Algadarif State – Sudan. Seed samples were taken randomly, treated with H2SO4 and placed in moist filter papers for germination. Roots tips were collected after three days for var. seyal and four days for var. fistula in the morning and in midday. They were treated with potassium chloride before fixation in aceto-alcohol. The prepared root tips were then sliced by microtome set at 6 um and were dyed with safranine and fast green and then prepared for microscopic examination by squash and permanent methods. The results showed that roots which are collected at midday showed active mitotic divisions compared to those collected in the morning. Also, pretreatment with potassium chloride resulted in full spread of the chromosoms. The karyotyping showed the chromosome number for var. seyal is tetraploid (2n=4x=52) and that of var. fitula is octaploid (2n=8x=104). The chromosomes were ranked from 1 to 26 for var. seyal according to the total length that varies from 5.60 µm to 2.68, with significant variation among them. Also, var. fistula chromosomes were ranked fom 1 to 52 according to the total length that ranged from 6.71 to 2.15, with significant variation among them. According to the centromere position, the haploid of chromosomes was classified as (4 median, 11 sub median and 11 slightly sub median) for var. seyal and (17 median, 17 sub median and 18 slightly sub median) for var fistula. The karyotyping results indicate clear differences between the two varieties and point the possibility that var. seyal was the progenitor of var. fistula.
Description: 58page
URI: http://khartoumspace.uofk.edu/handle/123456789/18841
Date: 2016-02-09


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