University of Khartoum

Towards A Noninvasive Approach to Malaria: Detection of Parasite DNA in Body Secretions and Surface Mucosa

Towards A Noninvasive Approach to Malaria: Detection of Parasite DNA in Body Secretions and Surface Mucosa

Show full item record

Title: Towards A Noninvasive Approach to Malaria: Detection of Parasite DNA in Body Secretions and Surface Mucosa
Author: El Rayah, El Amin; M.E, AbdelGayoum S.; H.A, Giha
Abstract: Invasive procedures for diagnostic or therapeutic purposes bear a relative risk of transmission of serious blood-borne infectious disease. In this study, a noninvasive approach to malaria diagnosis using polymerase chain reaction (PCR) for the detection of parasite DNA in saliva, buccal mucosa and urine (alternative samples) was examined. Saliva, buccal mucosa and urine samples were collected simultaneously with blood samples from 93 patients with microscopically confirmed Plasmodium falciparum infection. Species-specific primers detected the parasite DNA only in blood samples. However, when the PCR analysis was repeated using MSP1 and MSP2 primers in a subgroup of 21 complete sets of samples, the parasite DNA was detected in all except 3 samples, which were found to be negative with the MSP2 primers. Parasite density, body temperature or patient age did not influence the PCR results. In conclusion, P. falciparum parasite DNA was detected equally in saliva, buccal mucosa and urine of malaria patients, regardless of their ages, body temperatures or parasite density. Surprisingly, the parasite DNA was not amplified by species-specific primers in the alternative samples whereas it was in the blood samples.
URI: http://khartoumspace.uofk.edu/handle/123456789/19426
Date: 2016-02-25


Files in this item

Files Size Format View

This item appears in the following Collection(s)

Show full item record

Share

Search DSpace


Browse

My Account