University of Khartoum

Development of Derivative Spectrophotometric and HPLC Methods for Determination of Cefquinome sulphate and Niclosamide

Development of Derivative Spectrophotometric and HPLC Methods for Determination of Cefquinome sulphate and Niclosamide

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Title: Development of Derivative Spectrophotometric and HPLC Methods for Determination of Cefquinome sulphate and Niclosamide
Author: Shantier, Shaza Wagiealla
Abstract: Simple, sensitive and accurate spectrophotometric and HPLC methods were developed for the determination of cefquinome sulphate (CS) and niclosamide (NA) in bulk and dosage forms. The spectrophotometric method was based on the measurement of the first and second derivative spectra for the aqueous solution of cefquinome sulphate and the first derivative of the niclosamide methanolic solution at λmax 286nm, 300nm and 351nm, respectively. The HPLC-separation was conducted on Shimpac C-18 (25x4.6mm) column using suitable mobile phases consisted of 80:20 v/v ammonium acetate: acetonitrile and 70: 30 v/v methanol: water for the elution of cefquinome sulphate and niclosamide respectively. System suitability was assessed by measurement of factors affecting column efficiency. Beer’s law was applied over the concentration range 2-12μg/ml with a correlation coefficient not less than 0.999. The added recovery results were 100.1± 0.575% (n=3) for CS and 100.80 ± 0.59 (n=3) for NA, which indicates the absence of interference by the suspension and tablets excipients. The results obtained by the developed methods were statistically compared with those of reported methods and evaluated at 95% confidence limits.
URI: http://khartoumspace.uofk.edu/handle/123456789/19602
Date: 2015


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