University of Khartoum

Sexual Dimorphism Of Rat Submandibular Salivary Proteinases: A Histochemical And Biochemical Study Using Oligopeptide Substrates

Sexual Dimorphism Of Rat Submandibular Salivary Proteinases: A Histochemical And Biochemical Study Using Oligopeptide Substrates

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Title: Sexual Dimorphism Of Rat Submandibular Salivary Proteinases: A Histochemical And Biochemical Study Using Oligopeptide Substrates
Author: Garrett, J. R.; Proctor, G. B.; Shori, D. K.; Suliman, Ahmed M.; Clarke, G. A.
Abstract: Using a locally bred strain of Wistar rats, the proteinases in submandibular glands have be -n assessed histochemically and biochemically by their amidolytic activities with oligopeptide substrates, with the pur¬pose of testing for differences in male and female glands. Proteinase histochemistry with the cl- romogenic substrate D-Val-Leu-Arg-4-methoxy-2-naphthylamide (MNA) produced a strong reaction withi t secretory granules in the granular tubules and striated ducts and gave the clear impression that fewer granular tubules occur in female glands. This was confirmed morphometrically using DMAB staining of the f ranules in paraffin sections. The volume density in female glands was approximately 75% of that in mile glands, which appears not to have been observed in previous work by others. For biochemical testing the fluorogenic substrates D-Val-Leu-Arg-7-amino-4-trifiuoromethylcoumarin (AFC) alone or with soya bean trypsin inhibitor and Z-Val-Lys-Lys-Arg-AFC with or without aprotinin were used. In relatio i to DNA, it was found that the female glands contained only about 56% of the overall proteinase activity c f that pre¬sent in male glands. Tissue kallikrein in female glands was found to be approximately 72% of hat in the males when related to DNA and that for tonin was about 66%. Further testing of compositional cifferences was performed after separating the individual proteinases by isoelectric focussing. Their location was iden¬tified by applying a membrane overlay impregnated with the fluorogenic substrate D-Val-Leu- krg-AFC. This enabled precise excision of individual bands in the gels, which were then eluted and assa red quan¬titatively. In this way tissue kallikrein in the female glands was found to be about 85% of thr.t in male glands, whereas T-kininogenase and esterase A were only about half of that in the male. The re sults sup¬port the concept that male sex hormones exert a greater effect on the synthesis of T-kininog,-nase and esterase A than on tissue kallikrein.
URI: http://khartoumspace.uofk.edu/handle/123456789/19678
Date: 2016-03-14


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