University of Khartoum

Antimicobial Activity and Formulation of Cajanus cajan Leaves Tablets

Antimicobial Activity and Formulation of Cajanus cajan Leaves Tablets

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Title: Antimicobial Activity and Formulation of Cajanus cajan Leaves Tablets
Author: Mohammed, Aminah Mohammed Abedelgahyoum
Abstract: Background: Cajanus cajan plant is an erect shrub grows widely in all parts of the Sudan, and it is cultivated for many reasons such as fence, green leaves and tops are used as fodder. Dried fruits are commonly used as source of protein and leaves used traditionally for medicinal purpose. The aim of this study was to screen the antimicrobial activity of the leaves to find the best method of extraction, using different extracting solvents then formulate it in a suitable dosage form(as tablet). Method: Hot continuous extraction method using soxhelt and methanol 80% was selected among the other methods, then the methanolic extract of Cajanus cajan leaves was subjected to phytochemical screening tests, assay development tests, and antibacterial activity tests. Then, using wet granulation method to formulate it as tablets; three tablets formulas were prepared; using different concentrations of fillers. Accelerated stability testes after three months and six months were carried out. After that one of the three optimized formulas, was used to study the effect of binder type and concentration on its properties. Three different binder types (Povidone (P), Arabic Gum (A) and Guar gum (G), with three different concentrations 3%, 5% and 7%, and in constant amount of two types of superdisintegrants Sodium starch glycolate (SSG) and Cross carmellose (CC) were selected. Results: Phytochemical screening tests showed the presence of coumarin, tannins, triterpens, alkaloids, flavonoids and terrace of saponin. These results were confirmed by TLC, IR, HPLC and GC-MS results. The selected extract showed positive antibacterial activity against gram positive bacteria Staphylococcus aureus and Bacillus subtilis and against gram negative bacteria Escherichia coli and Pseudomonas aeruginosa. Antifungal activity against Aspergillus niger and Candida albicans was shown. More over when extract was mixed with standard antibiotics (Amikacin, Clindamycin, Gentamicin, and Ciprofloxacin) in the same media, they gave clear increased in inhibition zones and no significant changes occurred. The physicochemical properties (hardness, friability, disintegration time and dissolution tests) were evaluated for all formulated tablets. Cross carmellose showed marked decrease in the disintegration time (DT) than Sodium starch glycolate (SSG), in the presence of different binders with different concentrations. Povidone (P), showed gradual increase in disintegration time with the increase of concentration, hardness increased and the percent loss of friability was decreased. Whilst in Guar gum (GG) the disintegration time decreased with increasing of its concentration. Acacia (gum Arabic A) showed relatively long DT compared to Povidone and Guar Gum, but within the limits and relatively high loss percent, and an increased in the hardness with increased of its concentration. Dissolution profiles for all different formulas were studied, and the similarity factor f2, was used for the comparison between the formulas, all the values were above 50, except between the two formulas containing 3% and 7% Guar gum, which showed significant difference (f2 less than 50). Conclusion: Cajanus cajan leaves extract showed antibacterial activity, and it formulated into tablets with acceptable properties. The results suggest that, Guar gum and Acacia (gum Arabic) could be useful as an alternative binding agent for povidone to produce tablets with good properties and there was considerable interaction between the variables employed on the tablet properties.
Description: 149 Pages
URI: http://khartoumspace.uofk.edu/handle/123456789/19918
Date: 2016-03-22


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