University of Khartoum

In vitro activity of the aqueous extract of Gardenia ternifolia fruits against Theileria lestoquardi

In vitro activity of the aqueous extract of Gardenia ternifolia fruits against Theileria lestoquardi

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Title: In vitro activity of the aqueous extract of Gardenia ternifolia fruits against Theileria lestoquardi
Author: Hassan, Shawgi Mohamed; Farah,Hayat Mahgoub;etal
Abstract: The aim of this study is to screen the aqueous extract of Tinospora bakis roots for the discovery of its in vitro activity against Theileria lestoquardi. Lymphocyte cells infected with T.lestoquardi were isolated from heparinized blood with Ficoll-paque .These cells were grown in minimum essential medium; and the parasite was identified by indirect fluorescent antibody test. In vitro screening of the extract against T. lestoquardi which is the causative agent of malignant theileriosis in sheep showed activity of 17.66 and 30.00% at concentrations of 5000 and 10000 ppm, respectively. Concentrations of 250 and 500 ppm had no activity. Lethal concentration 50% (LC50) was 184268.54 ppm. The plant extract significantly (P<0.05) decreased the number of macroschizonts per cell, number of dividing cells (binucleated and multinucleated) and mean number of viable cells at concentrations of 5000 and 10000 ppm. However, the number of cells with extra cellular macroschizonts did not significantly (P˃0.05) increase. The extract also brought about a slight cytotoxicity at concentration of 10000 ppm. From this study it is concluded that the plant aqueous extract showed activity against T.lestoquardi and in vivo studies to confirm these results are needed to use the extract for the treatment of malignant ovine theileriosis
URI: http://khartoumspace.uofk.edu/handle/123456789/20513
Date: 2016-04-17


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