University of Khartoum

Molecular detection of Babekir (BK) Viruse in Sudanese kidney Transplant Recipients in Khartoum state

Molecular detection of Babekir (BK) Viruse in Sudanese kidney Transplant Recipients in Khartoum state

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Title: Molecular detection of Babekir (BK) Viruse in Sudanese kidney Transplant Recipients in Khartoum state
Author: Alamin, Ibrahim Awad Helibi
Abstract: Background: Establishing instant diagnosis of BKV is of great value for monitoring the patients, who are at possible risk for the development of BKV nephropathy. PCR method is a useful tool for the rapid and sensitive detection of reactivated BKV infection. This study was carried out to detect human polyomavirues virus (BKV) in renal transplant recipients in Khartoum State using urine cytology and detection of viral nucleic acid in urine and blood using conventional PCR method. Moreover, the identify of BK virus was confirmed by DNA sequencing for the first time in Sudan The aim of study was to establish molecular detection of BK viruse in Sudanese kidney transplant recipients in Khartoum state. Methods: A total of 100 plasma and urine samples were collected randomly from renal transplant recipients at Sudanese Renal Transplantation Center from September 2012 to March 2013. The samples were subjected to polymerase chain reaction assay to detect viral DNA, urine cytology to identified viral inclusion (decoy cell), and nucleotide sequence and phylogentic analysis of BKV based on T.large gene. Results: Among the 100 renal transplant patients, three revealed that BK virus cytological changes showed nuclear inclusions suspected to be associated with BK polyoma virus infection. According to the PCR, the BK virus nucleic acid were detected in 32 urine specimens (32%) while only 6 (6%) of BK virus nucleic acid were detected in plasma specimens. Successful sequence of BKV based on T large gene were obtained , by Macrogen Company Korea, to confirm the results on 6 positive PCR urine specimens, the results showed 3 were BKV, and the rest were JCV by nucleotide BLAST(http: //blast.ncbi.nlm.nih.gov/Blast.cgi). Phylogenetics analysis showed that the identity of BKV isolated in Sudan is 100% with Iran isolate (Gen Bank accession number ACY78383) and 99% with other isolated from South Africa (Gen Bank accession number BAF029), and Ethiopia (Gen Bank accession number BAF42908). Conclusion: Human polyomavirus BK is related to different clinical manifestations among renal transplant patients. The routine use of PCR on urine and plasma by PCR is a useful tool for the rapid and sensitive detection of reactivated BKV in asymptomatic recipients. Thus, establishing instant diagnosis may be of great value for monitoring the renal recipients, who are at possible risk for the development of BKV nephropathy.
Description: 61page
URI: http://khartoumspace.uofk.edu/handle/123456789/20888
Date: 2016-04-28


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