University of Khartoum

In Vitro Antifungal Activity And GC/MS Profiling Of Ten Selected Sudanese Essential Oils Against Madurella Mycetomatis, The Causative Agent Of Black-Grain Mycetoma

In Vitro Antifungal Activity And GC/MS Profiling Of Ten Selected Sudanese Essential Oils Against Madurella Mycetomatis, The Causative Agent Of Black-Grain Mycetoma

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Title: In Vitro Antifungal Activity And GC/MS Profiling Of Ten Selected Sudanese Essential Oils Against Madurella Mycetomatis, The Causative Agent Of Black-Grain Mycetoma
Author: Khalid, Sami A.
Abstract: Madurella mycetomatis is one of the most prevalent causative agents of black-grain eumycetoma. Infection caused by this fungus is notoriously difficult to treat due to absence of adequate therapy and frequently leads to surgical excision of the infected limb. Prolonged follow-up after surgery with currently available antifungals, however, might improve the clinical outcome [1]. Nevertheless, the increasing recurrence and resistance of these pathogenic organisms to existing antifungals warrant the development a reproducible in vitro assay for discovery of novel bioactive agents [2]. The new 96-well microplate's assay based on resazurin dye, which was developed by our group to assess the antifungal activity against Madurella mycetomatis [3] is presently applied to 10 essential oils. GC/MS analysis was carried out with an HP 5890 Series II gas chromatograph (FID) and the identification of the compounds was based on comparisons with published MS data and a computer library search. In vitro, M. mycetomatis appears to be highly susceptible to essential oils, hence the MICs of the essential oils ranged between 0.625 – 2.5 mg/mL, while the standard antifungal, itraconazole exhibited MIC of 0.354 IM. More than 300 mono- and sesquiterpenes were identified and a correlation between the bioactivity and the identified compound has been hypothesised.
URI: http://khartoumspace.uofk.edu/123456789/21167


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