University of Khartoum

Rat Stem-Cell Leukemia Gene Expression Increased during Testis Maturation

Rat Stem-Cell Leukemia Gene Expression Increased during Testis Maturation

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Title: Rat Stem-Cell Leukemia Gene Expression Increased during Testis Maturation
Author: Khalid, Ahmed; Asano, Atsushi; Ohyama, Kenji; Ohta, Masanori
Abstract: We found that stem-cell leukemia (SCL), also known as T cell acute-lymphocytic leukemia (Tal-1) gene expression, was upregulated in the maturing rat testis. Strong expression of Tal-1 was detected in the normal maturing rat testis by Northern blotting. Western blotting revealed the protein size to be about 34 kDa. Protein expression was wide-spread in spermatocytes, spermtids and spermatogonia in accordance with the seminiferous epithelium cycle, as determined by an analysis of immunohistochemistry. Gene expression of Tal-1 regulatory gene, NKX3.1, was negatively correlated with Tal-1 expression. Human Tal-1 expression in the maturing testis as well as in bone marrow was observed, which suggests that the gene product is a novel cancer-testis antigen candidate. Taken together, TAL-1 may be involved in cell division, morphological changes, and the development of spermatogenic cells in the normal rat testis.
URI: http://khartoumspace.uofk.edu/123456789/21486


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