University of Khartoum

Raising of Antiserum and Development of IRMA Serum Ferritin

Raising of Antiserum and Development of IRMA Serum Ferritin

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Title: Raising of Antiserum and Development of IRMA Serum Ferritin
Author: Abdalla, Omer M.; Elbagir, NabilaM.; Ali, Nagi I.
Abstract: Antiserum to human liver ferritin was developed by immunizing sheep with purified human liver ferritin. This antiserum has been purified using ammonium sulphate. A part of it was linked chemically to magnetisable particles, while the other part was adsorbed physically onto polystyrene beads in order to develop two IRMAs. The anti-ferritin antibody obtained was purified and diluted 200,000 folds before being coated to polystyrene beads, or coupled to magnetisable particles. Assay validation, sensitivity and accuracy tests for the two IRMAs were performed. The polystyrene beads IRMA system showed better performance than the magnetisable particles system. It was found that, the minimum detectable dose in the bead system was 0.6 ng /ml, whereas it was 6.6 ng/ml in the magnetisable one. In the beads system, the mean recovery of ferritin was found to be 98.5 % while the linearity tests showed a correlation coefficient of 0.996. The comparison between our coated beads IRMA with NETRIA's IRMA serum ferritin showed a correlation coefficient of 0.982.
URI: http://khartoumspace.uofk.edu/123456789/21790


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