University of Khartoum

A high performance liquid chromatography (HPLC) method with evaporative light scattering detector for quantififi cation of major phospholipids classes of donkey serum

A high performance liquid chromatography (HPLC) method with evaporative light scattering detector for quantififi cation of major phospholipids classes of donkey serum

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dc.contributor.author Seri, Hisham I.
dc.contributor.author Naim, Hassan Y.
dc.contributor.author Hassan, T.
dc.contributor.other Department of Pharmacology and Toxicology en_US
dc.date 2010
dc.date.accessioned 2016-06-23T10:15:48Z
dc.date.available 2016-06-23T10:15:48Z
dc.date.submitted 2016-06-23
dc.identifier.uri http://khartoumspace.uofk.edu/123456789/22167
dc.description.abstract An HPLC method with an evaporative laser scattering detector was used to quantify major phospholipids fractions in donkeys’ serum. Blood samples were collected bimonthly for a whole year from 20 donkeys (10 male and 10 female) from the Sudanese breed kept at the premises of the Central Veterinary Research Laboratory (CVRL), Soba. The method used made the excellent separation possible of phosphatidylglycerine (PG), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylcholine (PC), and sphingomyelin (SM). in 27 minutes, including the regeneration of the column. The SM resulted in two peaks (saturated and unsaturated fatty acids), as described by other researchers. The method was unable to separate phosphatidylserine (PS), which appeared in one peak with phosphatidylinositol. There is a signififi cant difference in the level of PG, PE, PI and SM between females and males.The method fully discussed in this study and the obtained values of major phospholipids can be used for health control and diagnosis of diseases. en_US
dc.publisher UOFK en_US
dc.relation.ispartofseries VETERINARSKI ARHIV 80 (3), 365-373, 2010
dc.subject donkeys en_US
dc.subject ELSD en_US
dc.subject HPLC en_US
dc.subject phospholipid en_US
dc.subject sphingolipid en_US
dc.subject sudan en_US
dc.title A high performance liquid chromatography (HPLC) method with evaporative light scattering detector for quantififi cation of major phospholipids classes of donkey serum en_US
dc.type Article en_US
dc.Faculty Veterinary en_US

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