University of Khartoum

Isolation and Characterization of 3-N-Trimethylamino-1Propanol Degrading Arthrobacter sp. Strain E5

Isolation and Characterization of 3-N-Trimethylamino-1Propanol Degrading Arthrobacter sp. Strain E5

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Title: Isolation and Characterization of 3-N-Trimethylamino-1Propanol Degrading Arthrobacter sp. Strain E5
Author: Ahmed, Isam A. Mohamed; Mori, Nobuhiro; etal
Abstract: The aim of this study was to screen for microorganism that able to utilize 3-N-Mmethylamino- 1-propanol (homocholine) as sole source of carbon and nitrogen and to see which mechanism is followed in the degradation of this compound by soil microorganisms. A gram-positive bacterium, designated, as strain E5 was isolated from soil. The strain was identified as Arthrobacter sp. strain E5 based on the phenotypic features, physiologic and biochemical characteristics and phylogenetic analysis. The cells of strain E5 displayed prim, branching at the exponential phase and fragmented into irregular rod and coccoid elements at the station, phase. The colonies were yellow th color, convex, round and entire with smooth and regular margins on both homocholine and nutrient agar medium. Comparative 16S rDNA sequencing studies indicated that strain E5 fall into Arthrobacter nicotinovorans subclade where it fonus a monophyletic group with the type strains of Anhrobacter nicotinovorans and Arthrobacter histidinolovorans. Metabolites analysis by capill, electrophoresis and gas cluomatography-mass spectrometry showed trimethylamine as a major metabolite beside 13-alanine betaine and trimethylaminopropionaldehyde. Therefore, the possible degradation pathway of homocholine th Arthrobacter sp. strain E5 is through consequence oxidation of alcohol group (-OH) to aldehyde (-CHO) and acid (-COOH), respectively and thereafter cleavages of C-N bond providing tHmethylamine and alkyl chain.
URI: http://khartoumspace.uofk.edu/123456789/22258


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