Abstract:
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Leishmaniasis remains a serious health problem. The outcome
of Leishmania infection depends on the early innate
response. In this study, whole blood samples of 40 patients
with visceral leishmaniasis (VL), 10 leishmanin skin testnegative
(LST ve) controls and 10 leishmanin skin testpositive
(LST+ve) controls were stimulated by live L. donovani
promastigotes. Also, THP1 human cell line was infected
with L. donovani. The production of interleukin 10 (IL-10),
tumour necrosis factor alpha (TNF) and interferon gamma
(IFNG) cytokines was measured, and the expression of Tolllike
receptors (TLR2, TLR4 and TLR9) was done in the
blood samples and also in the THP1 cell line. IL-10 was
found to be higher in LST+ve controls compared with VL
patients. TNF was moderately produced with no variation
between patients, controls and THP1 cells. IFNG was higher
in LST+ve controls also in THP1 cells. TLR4 and TLR9
were found to be highly expressed in patients with VL.
L. donovani increases the expression of TLR4 and TLR9 in
patients with VL and TLR2 in THP1 cells, suggesting a
TLRs relation in induction of a mixed cytokine response.
TLR9 was markedly recognized by L. donovani DNA. |